Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LPT17_RS02280 Genome accession   NZ_CP099502
Coordinates   423745..424242 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain JL42     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418745..429242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPT17_RS02240 (LPT17_02240) - 418789..419775 (+) 987 WP_000161315.1 ROK family glucokinase -
  LPT17_RS02245 (LPT17_02245) - 419775..420104 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LPT17_RS02250 (LPT17_02250) - 420101..420724 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LPT17_RS02255 (LPT17_02255) comGA 420776..421750 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LPT17_RS02260 (LPT17_02260) comGB 421722..422792 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  LPT17_RS02265 (LPT17_02265) comGC 422806..423117 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LPT17_RS02270 (LPT17_02270) comGD 423095..423541 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  LPT17_RS02275 (LPT17_02275) comGE 423528..423827 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  LPT17_RS02280 (LPT17_02280) comGF 423745..424242 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  LPT17_RS02285 (LPT17_02285) - 424339..424485 (+) 147 WP_001789879.1 hypothetical protein -
  LPT17_RS02290 (LPT17_02290) - 424475..424999 (+) 525 WP_001015120.1 shikimate kinase -
  LPT17_RS02295 (LPT17_02295) gcvT 425158..426249 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  LPT17_RS02300 (LPT17_02300) gcvPA 426269..427615 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LPT17_RS02305 (LPT17_02305) gcvPB 427608..429080 (+) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=700148 LPT17_RS02280 WP_001825340.1 423745..424242(+) (comGF) [Staphylococcus aureus strain JL42]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=700148 LPT17_RS02280 WP_001825340.1 423745..424242(+) (comGF) [Staphylococcus aureus strain JL42]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97