Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LPT16_RS02300 Genome accession   NZ_CP099495
Coordinates   427858..428355 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain JL28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422858..433355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPT16_RS02260 (LPT16_02260) - 422902..423888 (+) 987 WP_000161315.1 ROK family glucokinase -
  LPT16_RS02265 (LPT16_02265) - 423888..424217 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LPT16_RS02270 (LPT16_02270) - 424214..424837 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LPT16_RS02275 (LPT16_02275) comGA 424889..425863 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LPT16_RS02280 (LPT16_02280) comGB 425835..426905 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  LPT16_RS02285 (LPT16_02285) comGC 426919..427230 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LPT16_RS02290 (LPT16_02290) comGD 427208..427654 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  LPT16_RS02295 (LPT16_02295) comGE 427641..427940 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  LPT16_RS02300 (LPT16_02300) comGF 427858..428355 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  LPT16_RS02305 (LPT16_02305) - 428452..428598 (+) 147 WP_001789879.1 hypothetical protein -
  LPT16_RS02310 (LPT16_02310) - 428588..429112 (+) 525 WP_001015120.1 shikimate kinase -
  LPT16_RS02315 (LPT16_02315) gcvT 429271..430362 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  LPT16_RS02320 (LPT16_02320) gcvPA 430382..431728 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LPT16_RS02325 (LPT16_02325) gcvPB 431721..433193 (+) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=700018 LPT16_RS02300 WP_001825340.1 427858..428355(+) (comGF) [Staphylococcus aureus strain JL28]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=700018 LPT16_RS02300 WP_001825340.1 427858..428355(+) (comGF) [Staphylococcus aureus strain JL28]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97