NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	NG745_RS12040	Genome accession	NZ_CP099465
Coordinates	2472947..2474017 (-)	Length	356 a.a.
NCBI ID	WP_003153083.1	Uniprot ID	-
Organism	Bacillus velezensis strain Ag75
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2467947..2479017

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NG745_RS11990 (NG745_11990)	sipW	2468007..2468591 (-)	585	WP_015240205.1	signal peptidase I SipW	-
NG745_RS11995 (NG745_11995)	tapA	2468563..2469234 (-)	672	WP_063094776.1	amyloid fiber anchoring/assembly protein TapA	-
NG745_RS12000 (NG745_12000)	-	2469493..2469822 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
NG745_RS12005 (NG745_12005)	-	2469862..2470041 (-)	180	WP_003153093.1	YqzE family protein	-
NG745_RS12010 (NG745_12010)	comGG	2470098..2470475 (-)	378	WP_012117980.1	competence type IV pilus minor pilin ComGG	Machinery gene
NG745_RS12015 (NG745_12015)	comGF	2470476..2470976 (-)	501	WP_254895460.1	competence type IV pilus minor pilin ComGF	-
NG745_RS12020 (NG745_12020)	comGE	2470885..2471199 (-)	315	WP_080130386.1	competence type IV pilus minor pilin ComGE	Machinery gene
NG745_RS12025 (NG745_12025)	comGD	2471183..2471620 (-)	438	WP_012117983.1	competence type IV pilus minor pilin ComGD	Machinery gene
NG745_RS12030 (NG745_12030)	comGC	2471610..2471918 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
NG745_RS12035 (NG745_12035)	comGB	2471923..2472960 (-)	1038	WP_098081583.1	competence type IV pilus assembly protein ComGB	Machinery gene
NG745_RS12040 (NG745_12040)	comGA	2472947..2474017 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
NG745_RS12045 (NG745_12045)	-	2474210..2475160 (-)	951	WP_007408319.1	magnesium transporter CorA family protein	-
NG745_RS12050 (NG745_12050)	-	2475306..2476607 (+)	1302	WP_063094766.1	hemolysin family protein	-
NG745_RS12055 (NG745_12055)	-	2476645..2477475 (-)	831	WP_015240214.1	STAS domain-containing protein	-
NG745_RS12065 (NG745_12065)	-	2477682..2478062 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
NG745_RS12070 (NG745_12070)	-	2478299..2478544 (+)	246	WP_059368113.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.85 Da Isoelectric Point: 9.4729

                                >NTDB_id=699882 NG745_RS12040 WP_003153083.1 2472947..2474017(-) (comGA) [Bacillus velezensis strain Ag75]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=699882 NG745_RS12040 WP_003153083.1 2472947..2474017(-) (comGA) [Bacillus velezensis strain Ag75]

GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0699882