Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP78_RS28475 Genome accession   NZ_CP115253
Coordinates   6101824..6102447 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F033     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6096824..6107447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP78_RS28455 (PCP78_28445) aroB 6097419..6098525 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  PCP78_RS28460 (PCP78_28450) aroK 6098575..6099093 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PCP78_RS28465 (PCP78_28455) pilQ 6099105..6101249 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  PCP78_RS28470 (PCP78_28460) comL/pilP 6101303..6101827 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP78_RS28475 (PCP78_28465) pilO 6101824..6102447 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP78_RS28480 (PCP78_28470) pilN 6102444..6103040 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP78_RS28485 (PCP78_28475) pilM 6103040..6104104 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP78_RS28490 (PCP78_28480) - 6104289..6106760 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=699271 PCP78_RS28475 WP_003103268.1 6101824..6102447(-) (pilO) [Pseudomonas aeruginosa strain F033]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=699271 PCP78_RS28475 WP_003103268.1 6101824..6102447(-) (pilO) [Pseudomonas aeruginosa strain F033]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401