Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP61_RS19490 Genome accession   NZ_CP115231
Coordinates   3921339..3921962 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F051     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3916339..3926962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP61_RS19475 (PCP61_19465) - 3917026..3919497 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  PCP61_RS19480 (PCP61_19470) pilM 3919682..3920746 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP61_RS19485 (PCP61_19475) pilN 3920746..3921342 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP61_RS19490 (PCP61_19480) pilO 3921339..3921962 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP61_RS19495 (PCP61_19485) comL/pilP 3921959..3922483 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP61_RS19500 (PCP61_19490) pilQ 3922537..3924681 (+) 2145 WP_023083249.1 type 4a pilus secretin PilQ Machinery gene
  PCP61_RS19505 (PCP61_19495) aroK 3924693..3925211 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PCP61_RS19510 (PCP61_19500) aroB 3925261..3926367 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=697384 PCP61_RS19490 WP_003103268.1 3921339..3921962(+) (pilO) [Pseudomonas aeruginosa strain F051]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=697384 PCP61_RS19490 WP_003103268.1 3921339..3921962(+) (pilO) [Pseudomonas aeruginosa strain F051]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGTAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401