Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BVS141_RS12050 Genome accession   NZ_AP018402
Coordinates   2485772..2486842 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus velezensis strain S141     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2480772..2491842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVS141_RS12000 (BVS141_23790) sipW 2480832..2481416 (-) 585 WP_060562614.1 signal peptidase I SipW -
  BVS141_RS12005 (BVS141_23800) tapA 2481388..2482059 (-) 672 WP_060562615.1 amyloid fiber anchoring/assembly protein TapA -
  BVS141_RS12010 (BVS141_23810) - 2482318..2482647 (+) 330 WP_060562616.1 DUF3889 domain-containing protein -
  BVS141_RS12015 (BVS141_23820) - 2482687..2482866 (-) 180 WP_003153093.1 YqzE family protein -
  BVS141_RS12020 (BVS141_23830) comGG 2482923..2483300 (-) 378 WP_060562617.1 competence type IV pilus minor pilin ComGG Machinery gene
  BVS141_RS12025 (BVS141_23840) comGF 2483301..2483801 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  BVS141_RS12030 (BVS141_23850) comGE 2483710..2484024 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  BVS141_RS12035 (BVS141_23860) comGD 2484008..2484445 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  BVS141_RS12040 (BVS141_23870) comGC 2484435..2484701 (-) 267 WP_060562757.1 competence type IV pilus major pilin ComGC Machinery gene
  BVS141_RS12045 (BVS141_23880) comGB 2484748..2485785 (-) 1038 WP_060562618.1 competence type IV pilus assembly protein ComGB Machinery gene
  BVS141_RS12050 (BVS141_23890) comGA 2485772..2486842 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  BVS141_RS12055 (BVS141_23900) - 2487035..2487985 (-) 951 WP_119347867.1 magnesium transporter CorA family protein -
  BVS141_RS12060 (BVS141_23910) - 2488131..2489432 (+) 1302 WP_060562619.1 hemolysin family protein -
  BVS141_RS12065 (BVS141_23920) - 2489470..2490300 (-) 831 WP_015240214.1 STAS domain-containing protein -
  BVS141_RS12075 (BVS141_23940) - 2490507..2490887 (-) 381 WP_060562620.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BVS141_RS12080 (BVS141_23950) - 2491125..2491370 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=69519 BVS141_RS12050 WP_007408320.1 2485772..2486842(-) (comGA) [Bacillus velezensis strain S141]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=69519 BVS141_RS12050 WP_007408320.1 2485772..2486842(-) (comGA) [Bacillus velezensis strain S141]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTTCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGTTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment