Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NB630_RS13250 Genome accession   NZ_CP098255
Coordinates   2709072..2709569 (+) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain N4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704072..2714569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB630_RS13210 (NB630_13210) - 2704116..2705102 (+) 987 WP_000161314.1 ROK family glucokinase -
  NB630_RS13215 (NB630_13215) - 2705102..2705431 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NB630_RS13220 (NB630_13220) - 2705428..2706051 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NB630_RS13225 (NB630_13225) comGA 2706103..2707077 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NB630_RS13230 (NB630_13230) comGB 2707049..2708119 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  NB630_RS13235 (NB630_13235) comGC 2708133..2708444 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  NB630_RS13240 (NB630_13240) comGD 2708422..2708868 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  NB630_RS13245 (NB630_13245) comGE 2708855..2709154 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NB630_RS13250 (NB630_13250) comGF 2709072..2709569 (+) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  NB630_RS13255 (NB630_13255) - 2709666..2709812 (+) 147 WP_001789879.1 hypothetical protein -
  NB630_RS13260 (NB630_13260) - 2709802..2710326 (+) 525 WP_001015125.1 shikimate kinase -
  NB630_RS13265 (NB630_13265) gcvT 2710485..2711576 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  NB630_RS13270 (NB630_13270) gcvPA 2711596..2712942 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NB630_RS13275 (NB630_13275) gcvPB 2712935..2714407 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=694824 NB630_RS13250 WP_001794328.1 2709072..2709569(+) (comGF) [Staphylococcus aureus strain N4]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=694824 NB630_RS13250 WP_001794328.1 2709072..2709569(+) (comGF) [Staphylococcus aureus strain N4]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976