Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NB630_RS13225 Genome accession   NZ_CP098255
Coordinates   2706103..2707077 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain N4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2701103..2712077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB630_RS13190 (NB630_13190) rpmG 2701563..2701712 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  NB630_RS13195 (NB630_13195) - 2701921..2702460 (+) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  NB630_RS13200 (NB630_13200) - 2702472..2703935 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  NB630_RS13205 (NB630_13205) - 2703916..2704119 (+) 204 WP_000087561.1 YqgQ family protein -
  NB630_RS13210 (NB630_13210) - 2704116..2705102 (+) 987 WP_000161314.1 ROK family glucokinase -
  NB630_RS13215 (NB630_13215) - 2705102..2705431 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NB630_RS13220 (NB630_13220) - 2705428..2706051 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NB630_RS13225 (NB630_13225) comGA 2706103..2707077 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NB630_RS13230 (NB630_13230) comGB 2707049..2708119 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  NB630_RS13235 (NB630_13235) comGC 2708133..2708444 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  NB630_RS13240 (NB630_13240) comGD 2708422..2708868 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  NB630_RS13245 (NB630_13245) comGE 2708855..2709154 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NB630_RS13250 (NB630_13250) comGF 2709072..2709569 (+) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  NB630_RS13255 (NB630_13255) - 2709666..2709812 (+) 147 WP_001789879.1 hypothetical protein -
  NB630_RS13260 (NB630_13260) - 2709802..2710326 (+) 525 WP_001015125.1 shikimate kinase -
  NB630_RS13265 (NB630_13265) gcvT 2710485..2711576 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=694819 NB630_RS13225 WP_000697220.1 2706103..2707077(+) (comGA) [Staphylococcus aureus strain N4]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=694819 NB630_RS13225 WP_000697220.1 2706103..2707077(+) (comGA) [Staphylococcus aureus strain N4]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1