Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   MDRSPN_RS10630 Genome accession   NZ_AP018391
Coordinates   2011441..2012739 (-) Length   432 a.a.
NCBI ID   WP_000867608.1    Uniprot ID   A0A062WMW7
Organism   Streptococcus pneumoniae strain MDRSPN001     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2006441..2017739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDRSPN_RS10610 (MDRSPN_02015) rplI 2007596..2008048 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  MDRSPN_RS10615 (MDRSPN_02016) - 2008045..2010018 (-) 1974 WP_000715111.1 DHH family phosphoesterase -
  MDRSPN_RS10620 (MDRSPN_02017) hpf 2010154..2010702 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MDRSPN_RS10625 (MDRSPN_02018) comFC/cflB 2010782..2011444 (-) 663 WP_000649968.1 ComF family protein Machinery gene
  MDRSPN_RS10630 (MDRSPN_02019) comFA/cflA 2011441..2012739 (-) 1299 WP_000867608.1 DEAD/DEAH box helicase Machinery gene
  MDRSPN_RS10635 (MDRSPN_02020) - 2012795..2013430 (+) 636 WP_000395746.1 YigZ family protein -
  MDRSPN_RS10640 (MDRSPN_02021) cysK 2013715..2014635 (+) 921 WP_000029860.1 cysteine synthase A -
  MDRSPN_RS10650 (MDRSPN_02022) - 2014710..2015248 (-) 539 Protein_1989 transposase -
  MDRSPN_RS10655 (MDRSPN_02023) - 2015221..2015529 (+) 309 Protein_1990 transposase -
  MDRSPN_RS10660 (MDRSPN_02024) tnpB 2015670..2015993 (-) 324 WP_000973416.1 IS66 family insertion sequence element accessory protein TnpB -
  MDRSPN_RS10665 (MDRSPN_02025) tsf 2016239..2017279 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49590.50 Da        Isoelectric Point: 8.4382

>NTDB_id=69360 MDRSPN_RS10630 WP_000867608.1 2011441..2012739(-) (comFA/cflA) [Streptococcus pneumoniae strain MDRSPN001]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=69360 MDRSPN_RS10630 WP_000867608.1 2011441..2012739(-) (comFA/cflA) [Streptococcus pneumoniae strain MDRSPN001]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAAGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062WMW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae TIGR4

99.306

100

0.993
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment