Detailed information NTG0069347

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: MDRSPN_RS09710
• Genome accession: NZ_AP018391
• Coordinates: 1831013..1831474 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250545.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain MDRSPN001
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1826013..1836474

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MDRSPN_RS09680 (MDRSPN_01840)	-	1826324..1827148 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
MDRSPN_RS09685 (MDRSPN_01841)	rnpA	1827123..1827494 (-)	372	WP_000739246.1	ribonuclease P protein component	-
MDRSPN_RS11540	-	1827511..1827642 (-)	132	WP_000768904.1	hypothetical protein	-
MDRSPN_RS09690 (MDRSPN_01842)	-	1827643..1828833 (-)	1191	WP_000167766.1	acetate kinase	-
MDRSPN_RS09695 (MDRSPN_01843)	comYH	1828884..1829837 (-)	954	WP_000345119.1	class I SAM-dependent methyltransferase	Machinery gene
MDRSPN_RS09700 (MDRSPN_01844)	-	1829898..1830485 (-)	588	WP_000679774.1	class I SAM-dependent methyltransferase	-
MDRSPN_RS09705 (MDRSPN_01845)	comGG/cglG	1830622..1831035 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
MDRSPN_RS09710 (MDRSPN_01846)	comGF/cglF	1831013..1831474 (-)	462	WP_000250545.1	competence type IV pilus minor pilin ComGF	Machinery gene
MDRSPN_RS09715 (MDRSPN_01847)	comGE/cglE	1831437..1831739 (-)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
MDRSPN_RS09720 (MDRSPN_01848)	comGD/cglD	1831702..1832106 (-)	405	WP_000588005.1	competence type IV pilus minor pilin ComGD	Machinery gene
MDRSPN_RS09725 (MDRSPN_01849)	comGC/cglC	1832099..1832425 (-)	327	WP_000738650.1	comG operon protein ComGC	Machinery gene
MDRSPN_RS09730 (MDRSPN_01850)	comGB/cglB	1832427..1833443 (-)	1017	WP_013193332.1	competence type IV pilus assembly protein ComGB	Machinery gene
MDRSPN_RS09735 (MDRSPN_01851)	comGA/cglA/cilD	1833391..1834332 (-)	942	WP_000249567.1	competence type IV pilus ATPase ComGA	Machinery gene
MDRSPN_RS09740 (MDRSPN_01852)	-	1834408..1834773 (-)	366	WP_000286415.1	DUF1033 family protein	-
MDRSPN_RS09745 (MDRSPN_01853)	-	1834924..1835982 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17855.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=69347 MDRSPN_RS09710 WP_000250545.1 1831013..1831474(-) (comGF/cglF) [Streptococcus pneumoniae strain MDRSPN001]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=69347 MDRSPN_RS09710 WP_000250545.1 1831013..1831474(-) (comGF/cglF) [Streptococcus pneumoniae strain MDRSPN001]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915