Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NAG75_RS02475 Genome accession   NZ_CP097873
Coordinates   349746..350339 (+) Length   197 a.a.
NCBI ID   WP_005496571.1    Uniprot ID   A0A0F2HLP7
Organism   Vibrio parahaemolyticus strain GL-601     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344746..355339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG75_RS02460 - 345462..348032 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NAG75_RS02465 pilM 348175..349191 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NAG75_RS02470 pilN 349175..349753 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NAG75_RS02475 pilO 349746..350339 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAG75_RS02480 pilP 350329..350844 (+) 516 WP_025532853.1 pilus assembly protein PilP Machinery gene
  NAG75_RS02485 pilQ 350876..352618 (+) 1743 WP_208893477.1 type IV pilus secretin PilQ Machinery gene
  NAG75_RS02490 aroK 352807..353325 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NAG75_RS02495 aroB 353357..354457 (+) 1101 WP_017449465.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23019.57 Da        Isoelectric Point: 4.9419

>NTDB_id=693187 NAG75_RS02475 WP_005496571.1 349746..350339(+) (pilO) [Vibrio parahaemolyticus strain GL-601]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDGK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=693187 NAG75_RS02475 WP_005496571.1 349746..350339(+) (pilO) [Vibrio parahaemolyticus strain GL-601]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCATGATATCGGTCGTTTCTCACAGGCCATTGCCACACTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGGGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2HLP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711