Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   M8969_RS05315 Genome accession   NZ_CP097593
Coordinates   1059651..1061036 (+) Length   461 a.a.
NCBI ID   WP_032876163.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0229     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1054651..1066036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8969_RS05295 - 1055797..1056444 (-) 648 WP_007614107.1 YigZ family protein -
  M8969_RS05300 degS 1056667..1057830 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  M8969_RS05305 degU 1057907..1058596 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  M8969_RS05310 - 1058694..1059542 (+) 849 WP_032876160.1 DegV family protein -
  M8969_RS05315 comFA 1059651..1061036 (+) 1386 WP_032876163.1 DEAD/DEAH box helicase Machinery gene
  M8969_RS05320 - 1061094..1061378 (+) 285 WP_003151389.1 competence protein ComFB -
  M8969_RS05325 comFC 1061384..1062073 (+) 690 WP_196770545.1 phosphoribosyltransferase family protein Machinery gene
  M8969_RS05330 - 1062147..1062566 (+) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  M8969_RS05335 flgM 1062636..1062902 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  M8969_RS05340 - 1062917..1063399 (+) 483 WP_032876168.1 flagellar protein FlgN -
  M8969_RS05345 flgK 1063415..1064932 (+) 1518 WP_032876170.1 flagellar hook-associated protein FlgK -
  M8969_RS05350 flgL 1064944..1065861 (+) 918 WP_003151396.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52474.86 Da        Isoelectric Point: 9.9925

>NTDB_id=690841 M8969_RS05315 WP_032876163.1 1059651..1061036(+) (comFA) [Bacillus velezensis strain UA0229]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINGFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWASFAKRQIT
YCRACVMMGRADELTSLYSWNQASENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVVIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQLQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVIMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=690841 M8969_RS05315 WP_032876163.1 1059651..1061036(+) (comFA) [Bacillus velezensis strain UA0229]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAACGGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGTCCTTTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCTCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTCTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCGTGATAATAGATGAAGTAGATGCGTTTCCTTTTTCCGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACTGATTGGGTT
TGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCAGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCATCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATTGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(330-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.826

99.783

0.627
  comFA Latilactobacillus sakei subsp. sakei 23K

38.182

95.445

0.364