Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M8962_RS12585 Genome accession   NZ_CP097591
Coordinates   2590739..2591809 (+) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain UA0188     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2585739..2596809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8962_RS12555 - 2586208..2586453 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  M8962_RS12560 - 2586690..2587070 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M8962_RS12570 - 2587277..2588107 (+) 831 WP_012117987.1 STAS domain-containing protein -
  M8962_RS12575 - 2588145..2589446 (-) 1302 WP_012117986.1 hemolysin family protein -
  M8962_RS12580 - 2589592..2590542 (+) 951 WP_007408319.1 magnesium transporter CorA family protein -
  M8962_RS12585 comGA 2590739..2591809 (+) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  M8962_RS12590 comGB 2591796..2592833 (+) 1038 WP_012117984.1 competence type IV pilus assembly protein ComGB Machinery gene
  M8962_RS12595 comGC 2592838..2593146 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  M8962_RS12600 comGD 2593136..2593573 (+) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  M8962_RS12605 comGE 2593557..2593871 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  M8962_RS12610 comGF 2593780..2594280 (+) 501 WP_012117981.1 competence type IV pilus minor pilin ComGF -
  M8962_RS12615 comGG 2594281..2594658 (+) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  M8962_RS12620 - 2594715..2594894 (+) 180 WP_003153093.1 YqzE family protein -
  M8962_RS12625 - 2594934..2595263 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  M8962_RS12630 tapA 2595522..2596193 (+) 672 WP_012117978.1 amyloid fiber anchoring/assembly protein TapA -
  M8962_RS12635 - 2596165..2596749 (+) 585 WP_012117977.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=690792 M8962_RS12585 WP_012117985.1 2590739..2591809(+) (comGA) [Bacillus velezensis strain UA0188]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=690792 M8962_RS12585 WP_012117985.1 2590739..2591809(+) (comGA) [Bacillus velezensis strain UA0188]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTCTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374