Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   M8966_RS02300 Genome accession   NZ_CP097589
Coordinates   449755..451140 (-) Length   461 a.a.
NCBI ID   WP_012118573.1    Uniprot ID   A7Z9B6
Organism   Bacillus velezensis strain UA0311     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444755..456140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8966_RS02265 flgL 444930..445847 (-) 918 WP_012118569.1 flagellar hook-associated protein FlgL -
  M8966_RS02270 flgK 445859..447376 (-) 1518 WP_012118570.1 flagellar hook-associated protein FlgK -
  M8966_RS02275 - 447392..447874 (-) 483 WP_003151394.1 flagellar protein FlgN -
  M8966_RS02280 flgM 447889..448155 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  M8966_RS02285 - 448225..448644 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  M8966_RS02290 comFC 448718..449407 (-) 690 WP_197197403.1 double zinc ribbon domain-containing protein Machinery gene
  M8966_RS02295 - 449413..449697 (-) 285 WP_007407481.1 competence protein ComFB -
  M8966_RS02300 comFA 449755..451140 (-) 1386 WP_012118573.1 DEAD/DEAH box helicase Machinery gene
  M8966_RS02305 - 451248..452096 (-) 849 WP_003151387.1 DegV family protein -
  M8966_RS02310 degU 452194..452883 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  M8966_RS02315 degS 452960..454123 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  M8966_RS02320 - 454346..454993 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52493.84 Da        Isoelectric Point: 10.2968

>NTDB_id=690605 M8966_RS02300 WP_012118573.1 449755..451140(-) (comFA) [Bacillus velezensis strain UA0311]
MSEGASEFSEVRDFFFGRHLLRSEVPFSDHHIKRVTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVVMGRADELTSLYSWNQAHENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSHLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRNGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITSGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=690605 M8966_RS02300 WP_012118573.1 449755..451140(-) (comFA) [Bacillus velezensis strain UA0311]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTTCGGCCGGCATTTATTGAGATCTGAGGTACCGTT
TTCAGATCATCACATCAAGCGTGTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCGTGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCATGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACATCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAAACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTCACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATCACAAGCGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z9B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62