Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   M8286_RS02145 Genome accession   NZ_CP097577
Coordinates   428178..429470 (+) Length   430 a.a.
NCBI ID   WP_275601344.1    Uniprot ID   -
Organism   Streptococcus suis strain 3112     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423178..434470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8286_RS02120 (M8286_02130) - 423983..424624 (+) 642 WP_358638919.1 response regulator transcription factor -
  M8286_RS02125 (M8286_02135) - 424674..426074 (+) 1401 WP_029173958.1 Cof-type HAD-IIB family hydrolase -
  M8286_RS02130 (M8286_02140) - 426076..426447 (+) 372 WP_002937017.1 S1 RNA-binding domain-containing protein -
  M8286_RS02135 (M8286_02145) cysK 426473..427399 (-) 927 WP_002937015.1 cysteine synthase A -
  M8286_RS02140 (M8286_02150) - 427489..428121 (-) 633 WP_015646510.1 YigZ family protein -
  M8286_RS02145 (M8286_02155) comFA/cflA 428178..429470 (+) 1293 WP_275601344.1 DEAD/DEAH box helicase Machinery gene
  M8286_RS02150 (M8286_02160) - 429463..430128 (+) 666 WP_275601345.1 ComF family protein -
  M8286_RS02155 (M8286_02165) raiA 430205..430747 (+) 543 WP_275601346.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48969.42 Da        Isoelectric Point: 8.5948

>NTDB_id=690324 M8286_RS02145 WP_275601344.1 428178..429470(+) (comFA/cflA) [Streptococcus suis strain 3112]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQAVDNAVKEAGVQVFLTATSTDEL
DKKVRTGKLNRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLEKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIDFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSVERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=690324 M8286_RS02145 WP_275601344.1 428178..429470(+) (comFA/cflA) [Streptococcus suis strain 3112]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACGGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTATTGTCGAGCCTGCTTGCTTCTAGGCAGAGTGCGGTCAGACGAAAAACTCTATCATTTTCCCCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGATTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATTTACCACACCGTTGCCTCAG
TGATTGATAAAGGTGGAGCGGTTTGCCTAGCCAGTCCTCGAATCGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACTCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCAAGCAGTAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACGGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACGGGCAAATTAAACCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAATCCGCTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTCCCAAAGTTGGAAAAAGCGATTGAAGAAC
AGAGAAAGTCAGGCTTTCCCTTACTCATTTTTGTACCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGACTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CCAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGCAGGAGTGTGGAACGTCCGACTGGG
TTACTCCAGTTTTATCATGAAGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(291-393)

Type III restriction enzyme, res subunit (ResIII)

(91-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae Rx1

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae D39

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae R6

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae TIGR4

66.125

100

0.663
  comFA/cflA Streptococcus mitis SK321

65.661

100

0.658
  comFA Lactococcus lactis subsp. cremoris KW2

53.652

92.326

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.337

100

0.386
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363