Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M8120_RS11695 Genome accession   NZ_CP097576
Coordinates   2320449..2322464 (-) Length   671 a.a.
NCBI ID   WP_190359642.1    Uniprot ID   -
Organism   Microcystis aeruginosa str. Chao 1910     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315449..2327464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8120_RS11675 (M8120_11555) - 2315900..2316988 (-) 1089 Protein_2316 ISAs1 family transposase -
  M8120_RS11680 (M8120_11560) - 2317146..2317682 (-) 537 WP_190359645.1 hypothetical protein -
  M8120_RS11685 (M8120_11565) - 2317784..2318998 (-) 1215 WP_190359644.1 type II secretion system F family protein -
  M8120_RS11690 (M8120_11570) pilT 2319172..2320275 (-) 1104 WP_190359643.1 type IV pilus twitching motility protein PilT Machinery gene
  M8120_RS11695 (M8120_11575) pilB/pilB1 2320449..2322464 (-) 2016 WP_190359642.1 GspE/PulE family protein Machinery gene
  M8120_RS11700 (M8120_11580) - 2322987..2324909 (-) 1923 WP_190359641.1 S9 family peptidase -
  M8120_RS11710 (M8120_11590) - 2325207..2326412 (-) 1206 WP_190359640.1 FAD-dependent hydroxylase -
  M8120_RS11715 (M8120_11595) - 2326507..2327058 (-) 552 WP_190359639.1 phasin family protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74802.80 Da        Isoelectric Point: 5.5599

>NTDB_id=690295 M8120_RS11695 WP_190359642.1 2320449..2322464(-) (pilB/pilB1) [Microcystis aeruginosa str. Chao 1910]
MTFSSKKTRAVALPNYLSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQQLTGRPLPPDLQRQHKKNQLFELKIL
YGVESIDAELSDVDGLEIARLIDSLIPIDLCRRYRLIPLERLKGEPAAILIAMVDPDNLEASDDINRILRPKELGIQRLV
ITSEDYERLLEKFHRAQSQLEQEKARLEKEKELEKLSDITDIVGTIDVISNGPNDEVADDLGVGDANQAPVINLVNKIMA
KALQEGTSDIHIEPQEEFLKIRFRKDGVLYQAFEPLPKKITPSVTARLKIMADLDIAERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQNTLAIVRELASRPFGLLLVTGPTGSGKSTSLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLVRRVCTECRVAYHPTQEELARFGLAAANEREVTFYKANTLTIEEIQQARDQGNLCTKCG
GSGYKGRVGVYEVMQNSERLQQLINQGASTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLAEVERVTFTDSGLEAELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=690295 M8120_RS11695 WP_190359642.1 2320449..2322464(-) (pilB/pilB1) [Microcystis aeruginosa str. Chao 1910]
ATGACATTTTCTTCCAAGAAAACTCGTGCCGTTGCCCTGCCCAATTACCTTTCCCCCTTTGGTAATAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAACAGGCCCTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACCGGGCGCCCTTTGCCCCCGGATTTGCAGCGACAGCATAAGAAAAACCAGTTGTTTGAATTAAAAATTCTC
TACGGAGTCGAATCGATCGACGCCGAGTTGAGCGATGTAGATGGACTGGAAATAGCCCGGTTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGCCGCTATCGTCTCATCCCCCTGGAACGGCTCAAAGGGGAGCCGGCTGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTAGAGGCTAGCGACGATATTAATCGTATTCTCCGCCCCAAGGAGTTAGGTATACAGCGTTTAGTG
ATTACCAGCGAGGACTATGAAAGATTACTGGAAAAATTCCACCGGGCCCAATCGCAACTAGAACAGGAAAAAGCCCGACT
CGAAAAAGAAAAAGAACTGGAAAAACTCTCGGATATCACCGACATTGTCGGCACTATCGATGTGATCAGCAATGGCCCTA
ACGATGAAGTGGCCGATGATCTCGGGGTGGGGGACGCTAATCAAGCCCCCGTCATCAACCTGGTCAATAAAATTATGGCT
AAGGCCCTGCAGGAAGGCACTTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTTATCAAGCTTTTGAGCCGCTGCCGAAAAAAATTACCCCGTCGGTGACAGCCCGTTTGAAAATCATGGCCGATC
TCGATATCGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATGTATCAAGGGCGGAAAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGCGAAAAAGTCTGTTTGCGGATTCTCGATAACTCAGCTACCCAGTTGGGTTTAGATAA
ACTGATCACCGATCAAAATACCCTGGCAATTGTCCGGGAATTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGTC
CGACCGGTTCGGGGAAATCCACCAGTTTATACTCGGTTTTAGCCGAAAGAAATCACCCTGGAGTTAATATTAGTACCGCC
GAAGACCCGATCGAATATTCTCTACCAGGCATCACCCAGGTACAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTCCGGGCCTTCATGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAGACGGCCAAAACAGCGA
TCGAGGCGGCCTTAACTGGTCACTTGGTCTTAACTACCCTACACACTAACGATGCCGCCGGTGCGATCGCTCGCCTCGAT
GAAATGGGGGTTGAACCGTTTATGATTTCCGGTTCTCTGTTGGGAGTCTTGGCCCAGCGTCTGGTGCGGCGCGTCTGTAC
CGAGTGTCGAGTCGCCTATCACCCCACCCAAGAGGAATTAGCCCGTTTTGGTCTTGCCGCTGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTAACCATAGAGGAAATTCAACAGGCGCGAGATCAAGGTAATCTTTGTACCAAATGTGGT
GGCAGCGGTTATAAAGGTCGCGTCGGTGTCTATGAGGTAATGCAAAATAGTGAACGACTGCAGCAGTTAATTAACCAAGG
GGCAAGCACCGATCGCATTAAGGAGGCGGCCGTCGATGAGGGCATGGTGACACTCCTAGCTTACAGTTTAAATCTGGTGC
GAGAGGGTTACACCACCCTGGCAGAAGTGGAACGGGTGACATTTACCGATTCTGGTCTGGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
ACGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-178)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.356

98.659

0.714
  pilF Thermus thermophilus HB27

39.935

91.058

0.364