Detailed information NTG0690269

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M9A05_RS12175
• Genome accession: NZ_CP097575
• Coordinates: 2640904..2642052 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635904..2647052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M9A05_RS12155 (M9A05_12155)	-	2636642..2637826 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
M9A05_RS12160 (M9A05_12160)	-	2637823..2639196 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M9A05_RS12165 (M9A05_12165)	-	2639439..2639843 (-)	405	WP_003084560.1	NINE protein	-
M9A05_RS12170 (M9A05_12170)	czcD	2639997..2640896 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M9A05_RS12175 (M9A05_12175)	pilU	2640904..2642052 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M9A05_RS12180 (M9A05_12180)	pilT	2642230..2643264 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M9A05_RS12185 (M9A05_12185)	-	2643479..2644171 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M9A05_RS12190 (M9A05_12190)	proC	2644183..2645010 (+)	828	WP_250177733.1	pyrroline-5-carboxylate reductase	-
M9A05_RS12195 (M9A05_12195)	-	2645021..2645614 (+)	594	WP_003084544.1	YggT family protein	-
M9A05_RS12200 (M9A05_12200)	-	2645611..2645907 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=690269 M9A05_RS12175 WP_003100959.1 2640904..2642052(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF25]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=690269 M9A05_RS12175 WP_003100959.1 2640904..2642052(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF25]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGTGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382