NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	M5J22_RS12515	Genome accession	NZ_CP097326
Coordinates	2590500..2591114 (-)	Length	204 a.a.
NCBI ID	WP_069013538.1	Uniprot ID	-
Organism	Bacillus velezensis strain GUCC45
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2585500..2596114

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5J22_RS12485 (M5J22_12485)	rpsT	2585800..2586066 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M5J22_RS12490 (M5J22_12490)	holA	2586083..2587124 (-)	1042	Protein_2418	DNA polymerase III subunit delta	-
M5J22_RS12495 (M5J22_12495)	-	2587164..2587318 (-)	155	Protein_2419	hypothetical protein	-
M5J22_RS12500 (M5J22_12500)	-	2587359..2587493 (+)	135	WP_003152870.1	YqzM family protein	-
M5J22_RS12505 (M5J22_12505)	comEC	2587512..2589863 (-)	2352	WP_201489015.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M5J22_RS12510 (M5J22_12510)	-	2589864..2590433 (-)	570	WP_003152868.1	ComE operon protein 2	-
M5J22_RS12515 (M5J22_12515)	comEA	2590500..2591114 (-)	615	WP_069013538.1	helix-hairpin-helix domain-containing protein	Machinery gene
M5J22_RS12520 (M5J22_12520)	comER	2591173..2591994 (+)	822	WP_014305449.1	late competence protein ComER	-
M5J22_RS12525 (M5J22_12525)	-	2592063..2592800 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
M5J22_RS12530 (M5J22_12530)	rsfS	2592797..2593153 (-)	357	WP_003152864.1	ribosome silencing factor	-
M5J22_RS12535 (M5J22_12535)	yqeK	2593150..2593731 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M5J22_RS12540 (M5J22_12540)	-	2593721..2594290 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
M5J22_RS12545 (M5J22_12545)	yhbY	2594301..2594591 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
M5J22_RS12550 (M5J22_12550)	aroE	2594585..2595427 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22164.30 Da Isoelectric Point: 6.0433

                                >NTDB_id=688480 M5J22_RS12515 WP_069013538.1 2590500..2591114(-) (comEA) [Bacillus velezensis strain GUCC45]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=688480 M5J22_RS12515 WP_069013538.1 2590500..2591114(-) (comEA) [Bacillus velezensis strain GUCC45]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0688480