NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	M2M89_RS12630	Genome accession	NZ_CP097130
Coordinates	2478310..2478927 (-)	Length	205 a.a.
NCBI ID	WP_032726193.1	Uniprot ID	-
Organism	Bacillus subtilis strain S16
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2473310..2483927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2M89_RS12600	rpsT	2473611..2473877 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
M2M89_RS12605	holA	2473892..2474935 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
M2M89_RS12610	-	2474975..2475088 (-)	114	WP_122060479.1	hypothetical protein	-
M2M89_RS12615	yqzM	2475165..2475299 (+)	135	WP_003229983.1	YqzM family protein	-
M2M89_RS12620	comEC	2475340..2477670 (-)	2331	WP_128441738.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M2M89_RS12625	comEB	2477674..2478243 (-)	570	WP_003229978.1	ComE operon protein 2	-
M2M89_RS12630	comEA	2478310..2478927 (-)	618	WP_032726193.1	competence protein ComEA	Machinery gene
M2M89_RS12635	comER	2479011..2479832 (+)	822	WP_032726195.1	late competence protein ComER	-
M2M89_RS12640	yqeM	2479898..2480641 (-)	744	WP_032726197.1	class I SAM-dependent methyltransferase	-
M2M89_RS12645	rsfS	2480638..2480994 (-)	357	WP_003237112.1	ribosome silencing factor	-
M2M89_RS12650	yqeK	2481012..2481572 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M2M89_RS12655	nadD	2481562..2482131 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
M2M89_RS12660	yhbY	2482143..2482433 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
M2M89_RS12665	aroE	2482427..2483269 (-)	843	WP_041850031.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21753.50 Da Isoelectric Point: 4.8417

                                >NTDB_id=687444 M2M89_RS12630 WP_032726193.1 2478310..2478927(-) (comEA) [Bacillus subtilis strain S16]

MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=687444 M2M89_RS12630 WP_032726193.1 2478310..2478927(-) (comEA) [Bacillus subtilis strain S16]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376

Detailed information NTG0687444