Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M2M89_RS12185 Genome accession   NZ_CP097130
Coordinates   2397833..2398903 (-) Length   356 a.a.
NCBI ID   WP_070547533.1    Uniprot ID   -
Organism   Bacillus subtilis strain S16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2392833..2403903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M89_RS12140 tapA 2393311..2394072 (-) 762 WP_032726156.1 amyloid fiber anchoring/assembly protein TapA -
  M2M89_RS12145 yqzG 2394344..2394670 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  M2M89_RS12150 spoIIT 2394712..2394891 (-) 180 WP_014480252.1 YqzE family protein -
  M2M89_RS12155 comGG 2394963..2395337 (-) 375 WP_032726157.1 ComG operon protein ComGG Machinery gene
  M2M89_RS12160 comGF 2395338..2395721 (-) 384 WP_032726158.1 ComG operon protein ComGF Machinery gene
  M2M89_RS12165 comGE 2395747..2396094 (-) 348 WP_021480020.1 ComG operon protein 5 Machinery gene
  M2M89_RS12170 comGD 2396078..2396509 (-) 432 WP_038829732.1 comG operon protein ComGD Machinery gene
  M2M89_RS12175 comGC 2396499..2396795 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  M2M89_RS12180 comGB 2396809..2397846 (-) 1038 WP_077671432.1 comG operon protein ComGB Machinery gene
  M2M89_RS12185 comGA 2397833..2398903 (-) 1071 WP_070547533.1 competence protein ComGA Machinery gene
  M2M89_RS12190 - 2399131..2399313 (-) 183 WP_033884706.1 hypothetical protein -
  M2M89_RS12195 corA 2399315..2400268 (-) 954 WP_004399136.1 magnesium transporter CorA -
  M2M89_RS12200 yqhB 2400411..2401739 (+) 1329 WP_003230150.1 hemolysin family protein -
  M2M89_RS12205 rsbRD 2401792..2402628 (-) 837 WP_021480027.1 RsbT co-antagonist protein RsbRD -
  M2M89_RS12215 mgsR 2402852..2403232 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  M2M89_RS12220 yqgY 2403464..2403709 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40444.57 Da        Isoelectric Point: 8.8402

>NTDB_id=687440 M2M89_RS12185 WP_070547533.1 2397833..2398903(-) (comGA) [Bacillus subtilis strain S16]
MDSIENVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=687440 M2M89_RS12185 WP_070547533.1 2397833..2398903(-) (comGA) [Bacillus subtilis strain S16]
TTGGATTCAATAGAAAATGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCTGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCCATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997