Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LEUCM_RS05800 Genome accession   NZ_AP017929
Coordinates   1156680..1157192 (+) Length   170 a.a.
NCBI ID   WP_025016386.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1151680..1162192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEUCM_RS05760 (LACBS_01131) - 1151894..1152268 (-) 375 WP_011375004.1 hypothetical protein -
  LEUCM_RS05765 (LACBS_01132) - 1152365..1152865 (+) 501 WP_016265379.1 VanZ family protein -
  LEUCM_RS05770 (LACBS_01133) - 1152956..1153687 (+) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LEUCM_RS05775 (LACBS_01134) comGA 1153803..1154693 (+) 891 WP_025016380.1 competence type IV pilus ATPase ComGA Machinery gene
  LEUCM_RS05780 (LACBS_01135) comGB 1154686..1155693 (+) 1008 WP_056936423.1 type II secretion system F family protein Machinery gene
  LEUCM_RS05785 (LACBS_01136) comGC 1155690..1155989 (+) 300 WP_025016383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LEUCM_RS05790 (LACBS_01137) comGD 1155961..1156413 (+) 453 WP_025016384.1 competence type IV pilus minor pilin ComGD Machinery gene
  LEUCM_RS05795 (LACBS_01138) comGE 1156400..1156705 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  LEUCM_RS05800 (LACBS_01139) comGF 1156680..1157192 (+) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  LEUCM_RS05805 (LACBS_01140) - 1157155..1157505 (+) 351 WP_096695394.1 hypothetical protein -
  LEUCM_RS05810 (LACBS_01141) - 1157607..1158626 (+) 1020 WP_241652828.1 class I SAM-dependent methyltransferase -
  LEUCM_RS05815 (LACBS_01142) - 1158648..1159844 (+) 1197 WP_025016389.1 acetate/propionate family kinase -
  LEUCM_RS05820 (LACBS_01143) - 1159899..1160357 (+) 459 WP_025016390.1 hypothetical protein -
  LEUCM_RS05830 (LACBS_01144) - 1160900..1161265 (-) 366 WP_011374991.1 DUF805 domain-containing protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19690.57 Da        Isoelectric Point: 6.6472

>NTDB_id=68560 LEUCM_RS05800 WP_025016386.1 1156680..1157192(+) (comGF) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=68560 LEUCM_RS05800 WP_025016386.1 1156680..1157192(+) (comGF) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
TTGCGATTAATTGGACGACCGATTAAACACTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAGATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACATGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATCAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.824

100

0.988

Multiple sequence alignment