Detailed information NTG0685245

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2I82_RS02025
• Genome accession: NZ_CP096927
• Coordinates: 434290..435438 (+)
• Length: 382 a.a.
• NCBI ID: WP_224154891.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NY5506
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429290..440438

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I82_RS02000 (M2I82_02000)	-	430441..430737 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2I82_RS02005 (M2I82_02005)	-	430734..431327 (-)	594	WP_003084544.1	YggT family protein	-
M2I82_RS02010 (M2I82_02010)	proC	431338..432159 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2I82_RS02015 (M2I82_02015)	-	432171..432863 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2I82_RS02020 (M2I82_02020)	pilT	433078..434112 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2I82_RS02025 (M2I82_02025)	pilU	434290..435438 (+)	1149	WP_224154891.1	type IV pilus ATPase PilU	Machinery gene
M2I82_RS02030 (M2I82_02030)	czcD	435446..436345 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2I82_RS02035 (M2I82_02035)	-	436499..436903 (+)	405	WP_003084560.1	NINE protein	-
M2I82_RS02040 (M2I82_02040)	-	437146..438519 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M2I82_RS02045 (M2I82_02045)	-	438516..439700 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42529.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=685245 M2I82_RS02025 WP_224154891.1 434290..435438(+) (pilU) [Pseudomonas aeruginosa strain NY5506]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSREAMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685245 M2I82_RS02025 WP_224154891.1 434290..435438(+) (pilU) [Pseudomonas aeruginosa strain NY5506]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGGCCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.958	100	0.88
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60	91.623	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	88.743	0.38