Detailed information NTG0001376

Experimentally validated

Overview

• Name: sxy/tfoX
• Type: Regulator
• Locus tag: D1108_RS09055
• Genome accession: NZ_CP031859
• Coordinates: 1959911..1960567 (-)
• Length: 218 a.a.
• NCBI ID: WP_005609196.1
• Uniprot ID: A3N356
• Organism: Actinobacillus pleuropneumoniae strain HS143
• Function: positive regulator of competence gene Competence regulation

Function

We have identified a highly transformable strain of Actinobacillus pleuropneumoniae whose competence is regulated by the competence-activator Sxy as in other Pasteurellaceae.

Genomic Context

Location: 1954911..1965567

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D1108_RS09035 (D1108_09090)	nanA	1955285..1956163 (+)	879	WP_005599265.1	N-acetylneuraminate lyase	-
D1108_RS09040 (D1108_09095)	nagB	1956213..1957016 (+)	804	WP_005602606.1	glucosamine-6-phosphate deaminase	-
D1108_RS09045 (D1108_09100)	nagA	1957068..1958213 (+)	1146	WP_005599269.1	N-acetylglucosamine-6-phosphate deacetylase	-
D1108_RS09050 (D1108_09105)	fumC	1958362..1959756 (-)	1395	WP_005605803.1	class II fumarate hydratase	-
D1108_RS09055 (D1108_09110)	sxy/tfoX	1959911..1960567 (-)	657	WP_005609196.1	TfoX/Sxy family DNA transformation protein	Regulator
D1108_RS09060 (D1108_09115)	rpsJ	1960975..1961286 (+)	312	WP_001181005.1	30S ribosomal protein S10	-
D1108_RS09065 (D1108_09120)	rplC	1961312..1961938 (+)	627	WP_005599280.1	50S ribosomal protein L3	-
D1108_RS09070 (D1108_09125)	rplD	1961954..1962556 (+)	603	WP_005599282.1	50S ribosomal protein L4	-
D1108_RS09075 (D1108_09130)	rplW	1962553..1962858 (+)	306	WP_005599290.1	50S ribosomal protein L23	-
D1108_RS09080 (D1108_09135)	rplB	1962879..1963700 (+)	822	WP_005599291.1	50S ribosomal protein L2	-
D1108_RS09085 (D1108_09140)	rpsS	1963728..1964003 (+)	276	WP_005539416.1	30S ribosomal protein S19	-
D1108_RS09090 (D1108_09145)	rplV	1964014..1964346 (+)	333	WP_005599292.1	50S ribosomal protein L22	-
D1108_RS09095 (D1108_09150)	rpsC	1964365..1965072 (+)	708	WP_005599293.1	30S ribosomal protein S3	-
D1108_RS09100 (D1108_09155)	rplP	1965086..1965496 (+)	411	WP_005599294.1	50S ribosomal protein L16	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 25633.15 Da
• Isoelectric Point: 9.9359

>NTDB_id=1376 D1108_RS09055 WP_005609196.1 1959911..1960567(-) (sxy/tfoX) [Actinobacillus pleuropneumoniae strain HS143]
MAISPKKFQYLKEIFSPLGEINFKSYFSYLGIFKDDTMFALYDHKNDRLYLRKSAQFYPDIIRTIPIHFLIDRRIGKQQS
HIFYLIPSSIIHNLHLYTHWILSAIEEYQTAKAKLISQNKNKIRLLPNLNINIERLLARIEIYTVDDLKNVGVINAFVKL
IMLGLEVTELLLFKLYAALEHKYIYMLSKQEKQSLLIEADLSLYNAGLRKRFAISQAN

Nucleotide

• Length: 657 bp

>NTDB_id=1376 D1108_RS09055 WP_005609196.1 1959911..1960567(-) (sxy/tfoX) [Actinobacillus pleuropneumoniae strain HS143]
ATGGCAATATCCCCAAAAAAGTTCCAATATCTTAAGGAGATTTTTAGTCCTCTTGGAGAAATTAACTTCAAAAGCTATTT
TTCTTACTTAGGAATATTTAAAGACGATACTATGTTCGCCCTCTATGATCATAAAAATGATCGATTATACTTAAGAAAAT
CCGCTCAATTTTATCCGGATATTATAAGAACAATACCGATACATTTTTTAATTGATCGTCGTATCGGTAAGCAACAATCT
CATATTTTTTATCTTATACCTTCTTCTATTATTCACAATCTTCATTTATATACTCATTGGATTCTCTCTGCTATCGAAGA
ATATCAAACTGCAAAGGCCAAATTGATTTCTCAAAATAAAAATAAAATTCGTCTGCTTCCCAATTTGAATATCAATATAG
AAAGATTATTGGCACGTATTGAGATTTATACCGTAGATGATTTAAAAAACGTAGGCGTGATTAATGCGTTTGTAAAACTG
ATAATGCTAGGCTTGGAAGTAACCGAATTACTCCTCTTCAAACTCTACGCTGCGCTCGAACATAAATATATCTATATGTT
ATCCAAGCAAGAAAAACAATCCCTATTAATTGAAGCCGATTTATCTCTCTATAACGCAGGCCTACGTAAACGCTTCGCTA
TCTCACAGGCTAACTAA

Domains

Predicted by InterproScan.

• TfoX C-terminal domain (TfoX_C) (120-196)

• TfoX N-terminal domain (TfoX_N) (13-107)

3D structure

• AlphaFold DB: A3N356

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sxy/tfoX	Actinobacillus suis H91-0380	63.721	100	0.64
sxy/tfoX	Glaesserella parasuis strain SC1401	39.234	98.122	0.385

References

• [1]: Janine T Bossé et al. (2009) Natural competence in strains of Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 298(1):124-30. [PMID: 19659732]