Detailed information    

experimental Experimentally validated

Overview

Name   sxy/tfoX   Type   Regulator
Locus tag   A4U84_RS05295 Genome accession   NZ_CP015099
Coordinates   1161549..1162190 (+) Length   213 a.a.
NCBI ID   WP_021111957.1    Uniprot ID   A0A836MEW3
Organism   Glaesserella parasuis strain SC1401     
Function   positive regulator of competence gene   
Competence regulation

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 1156549..1167190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS05270 (A4U84_05270) - 1156599..1157633 (-) 1035 WP_062924130.1 methionine synthase -
  A4U84_RS05275 (A4U84_05275) - 1157656..1158633 (-) 978 WP_021114330.1 DUF1852 domain-containing protein -
  A4U84_RS05280 (A4U84_05280) ffh 1158940..1160322 (+) 1383 WP_010786427.1 signal recognition particle protein -
  A4U84_RS05290 (A4U84_05290) - 1160597..1161285 (+) 689 Protein_1031 ISAs1 family transposase -
  A4U84_RS05295 (A4U84_05295) sxy/tfoX 1161549..1162190 (+) 642 WP_021111957.1 TfoX/Sxy family DNA transformation protein Regulator
  A4U84_RS05300 (A4U84_05300) fumC 1162338..1163732 (+) 1395 WP_035523130.1 class II fumarate hydratase -
  A4U84_RS05305 (A4U84_05305) - 1163914..1165092 (+) 1179 WP_015939850.1 ATP-grasp domain-containing protein -
  A4U84_RS05310 (A4U84_05310) - 1165227..1165985 (+) 759 WP_021112874.1 esterase family protein -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24775.77 Da        Isoelectric Point: 9.7347

>NTDB_id=1391 A4U84_RS05295 WP_021111957.1 1161549..1162190(+) (sxy/tfoX) [Glaesserella parasuis strain SC1401]
MKYIDVKTQHIRNLLYPIIGETQAKTYFSYYGIMKDGALFALYKNSKFYLHISGNCLHEVLTNPNISQLSDSKYGINSKS
FYLIPTEILYDLKPISHWITNGIQEIINTKHTQYIKKKRCIRTLPNMTIQLERTLKKLGINSIDELIDRGEVDIFVNLLK
MGIDADQAILFRLYGAINRQYIYTISDKTKQDLLNDANHALYEAGLRKRFNVK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=1391 A4U84_RS05295 WP_021111957.1 1161549..1162190(+) (sxy/tfoX) [Glaesserella parasuis strain SC1401]
ATGAAATACATTGATGTAAAAACACAACACATTAGAAATTTGCTTTATCCTATAATTGGAGAAACTCAAGCAAAAACATA
TTTTTCCTACTATGGAATAATGAAAGATGGAGCTCTGTTTGCTCTATATAAAAATAGTAAATTCTATCTTCATATCTCAG
GAAACTGTCTACATGAAGTACTAACTAATCCCAATATATCCCAACTGTCCGACTCTAAATATGGTATCAATTCTAAATCT
TTCTATTTGATTCCAACAGAAATTTTATATGATTTAAAACCCATCTCCCATTGGATAACCAATGGTATTCAAGAGATTAT
AAATACAAAACACACCCAATATATAAAAAAGAAAAGATGTATCCGTACCTTACCTAATATGACTATTCAACTAGAAAGGA
CATTGAAAAAGTTAGGTATCAACTCCATCGATGAACTGATTGATAGAGGTGAAGTGGATATATTCGTTAATCTTCTTAAG
ATGGGTATAGATGCTGATCAAGCTATTTTGTTCCGTCTTTATGGAGCAATTAATCGACAATATATTTATACTATCTCTGA
TAAGACTAAGCAGGATCTACTTAATGATGCAAATCATGCTCTATATGAGGCTGGCTTACGTAAGCGCTTCAATGTTAAAT
AA

Domains

Predicted by InterproScan.

TfoX N-terminal domain (TfoX_N)

(14-105)

TfoX C-terminal domain (TfoX_C)

(120-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836MEW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sxy/tfoX Actinobacillus suis H91-0380

41.429

98.592

0.408
  sxy/tfoX Actinobacillus pleuropneumoniae strain HS143

39.234

98.122

0.385

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]