Detailed information NTG0001451

Experimentally validated

Overview

• Name: sxy/tfoX
• Type: Regulator
• Locus tag: ASU2_RS08740
• Genome accession: NC_018690
• Coordinates: 1901051..1901695 (+)
• Length: 214 a.a.
• NCBI ID: WP_015674037.1
• Uniprot ID: K0FZ56
• Organism: Actinobacillus suis H91-0380
• Function: positive regulator of competence gene Competence regulation

Function

Competence was reduced by addition of AMP in both species, suggesting that the mechanisms by which AMP regulates competence are conserved. Since A. pleuropneumoniae and A. suis are both distant relatives of H. influenzae, we conclude that purine nucleotide repression of Sxy translation is likely to be an ancestral property of the Pasteurellaceae.

Genomic Context

Location: 1896051..1906695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ASU2_RS08695 (ASU2_08540)	rplP	1896113..1896523 (-)	411	WP_005625160.1	50S ribosomal protein L16	-
ASU2_RS08700 (ASU2_08545)	rpsC	1896537..1897241 (-)	705	WP_014992367.1	30S ribosomal protein S3	-
ASU2_RS08705 (ASU2_08550)	rplV	1897260..1897592 (-)	333	WP_005599292.1	50S ribosomal protein L22	-
ASU2_RS11805	rpsS	1897603..1897877 (-)	275	Protein_1702	30S ribosomal protein S19	-
ASU2_RS08715 (ASU2_08565)	rplB	1897905..1898726 (-)	822	WP_014992368.1	50S ribosomal protein L2	-
ASU2_RS08720 (ASU2_08570)	rplW	1898747..1899052 (-)	306	WP_005599290.1	50S ribosomal protein L23	-
ASU2_RS08725 (ASU2_08575)	rplD	1899049..1899651 (-)	603	WP_005599282.1	50S ribosomal protein L4	-
ASU2_RS08730 (ASU2_08580)	rplC	1899667..1900293 (-)	627	WP_014992369.1	50S ribosomal protein L3	-
ASU2_RS08735 (ASU2_08585)	rpsJ	1900319..1900630 (-)	312	WP_001181005.1	30S ribosomal protein S10	-
ASU2_RS08740 (ASU2_08590)	sxy/tfoX	1901051..1901695 (+)	645	WP_015674037.1	TfoX/Sxy family DNA transformation protein	Regulator
ASU2_RS08745 (ASU2_08595)	rpsT	1901749..1902012 (-)	264	WP_005619152.1	30S ribosomal protein S20	-
ASU2_RS08750 (ASU2_08600)	murJ	1902419..1903987 (+)	1569	WP_015674038.1	murein biosynthesis integral membrane protein MurJ	-
ASU2_RS08755 (ASU2_08605)	-	1904009..1905076 (+)	1068	WP_015674039.1	MFS transporter	-

Sequence

Protein

• Length: 214 a.a.
• Molecular weight: 25045.35 Da
• Isoelectric Point: 9.8688

>NTDB_id=1451 ASU2_RS08740 WP_015674037.1 1901051..1901695(+) (sxy/tfoX) [Actinobacillus suis H91-0380]
MKYTTKELERLKALLSPLGEVTFKPYFSFLGIFKNDTMFALYKDQHLYVRKSAQYLEEITQTISIHFLVDRHINCRSKIF
YLLPSSILNNLQTYSHWITSAIHEYQQSKAKIENQNQNKIRMLPNLNISIERLLAKVGIYTVTDLKATGAINVFVRLIQQ
GLEATALLLFKLYSAIQYKYIYMLTEQEKQNLLIEADNALYQAGLRKRFMLCVK

Nucleotide

• Length: 645 bp

>NTDB_id=1451 ASU2_RS08740 WP_015674037.1 1901051..1901695(+) (sxy/tfoX) [Actinobacillus suis H91-0380]
ATGAAATACACAACAAAGGAGTTAGAAAGACTTAAAGCGCTATTGTCCCCTCTCGGGGAGGTGACCTTTAAGCCTTATTT
TTCATTCTTAGGTATCTTTAAAAACGATACCATGTTTGCCCTCTACAAAGATCAACATTTATATGTACGCAAATCAGCCC
AATATTTAGAAGAAATAACTCAAACAATATCGATTCATTTTCTGGTTGATCGTCATATCAACTGTCGTTCCAAAATATTT
TATCTTCTTCCTTCCTCAATTCTTAACAACCTACAAACTTACTCTCACTGGATCACTTCAGCCATTCACGAATATCAACA
ATCTAAAGCAAAAATTGAAAATCAAAATCAAAACAAAATTCGTATGTTACCAAACTTAAATATCAGTATTGAGAGATTAT
TGGCTAAAGTCGGTATTTATACAGTAACAGATTTAAAAGCGACAGGTGCTATTAACGTTTTTGTACGTTTAATCCAGCAA
GGTTTGGAAGCAACCGCATTGTTATTATTTAAACTATATAGTGCGATTCAATATAAATATATTTATATGCTGACAGAACA
AGAAAAACAGAATTTATTAATTGAAGCCGACAATGCGCTTTATCAAGCAGGATTACGGAAAAGATTCATGCTGTGCGTAA
AATAA

Domains

Predicted by InterproScan.

• TfoX C-terminal domain (TfoX_C) (117-193)

• TfoX N-terminal domain (TfoX_N) (14-104)

3D structure

• AlphaFold DB: K0FZ56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sxy/tfoX	Actinobacillus pleuropneumoniae strain HS143	63.721	100	0.64
sxy/tfoX	Glaesserella parasuis strain SC1401	41.429	98.592	0.408

References

• [1]: Sunita Sinha et al. (2013) The availability of purine nucleotides regulates natural competence by controlling translation of the competence activator Sxy. Molecular Microbiology 88(6):1106-19. [PMID: 23663205]