Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PALA35_RS28365 Genome accession   NZ_CP110346
Coordinates   6119715..6120338 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PALA35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6114715..6125338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA35_RS28345 (PALA35_05616) aroB 6115310..6116416 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  PALA35_RS28350 (PALA35_05617) aroK 6116466..6116984 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA35_RS28355 (PALA35_05618) pilQ 6116996..6119140 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  PALA35_RS28360 (PALA35_05619) comL/pilP 6119194..6119718 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PALA35_RS28365 (PALA35_05620) pilO 6119715..6120338 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PALA35_RS28370 (PALA35_05621) pilN 6120335..6120931 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PALA35_RS28375 (PALA35_05622) pilM 6120931..6121995 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PALA35_RS28380 (PALA35_05623) - 6122180..6124651 (+) 2472 WP_023126737.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=683909 PALA35_RS28365 WP_003103268.1 6119715..6120338(-) (pilO) [Pseudomonas aeruginosa strain PALA35]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=683909 PALA35_RS28365 WP_003103268.1 6119715..6120338(-) (pilO) [Pseudomonas aeruginosa strain PALA35]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401