Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M0696_RS12715 Genome accession   NZ_CP096590
Coordinates   2524099..2524716 (-) Length   205 a.a.
NCBI ID   WP_222143196.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2519099..2529716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS12685 (M0696_12685) rpsT 2519400..2519666 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  M0696_RS12690 (M0696_12690) holA 2519681..2520724 (-) 1044 WP_222143193.1 DNA polymerase III subunit delta -
  M0696_RS12695 (M0696_12695) - 2520764..2520913 (-) 150 WP_166850619.1 hypothetical protein -
  M0696_RS12700 (M0696_12700) - 2520954..2521088 (+) 135 WP_003229983.1 YqzM family protein -
  M0696_RS12705 (M0696_12705) comEC 2521129..2523459 (-) 2331 WP_222143194.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M0696_RS12710 (M0696_12710) - 2523463..2524032 (-) 570 WP_222143195.1 ComE operon protein 2 -
  M0696_RS12715 (M0696_12715) comEA 2524099..2524716 (-) 618 WP_222143196.1 helix-hairpin-helix domain-containing protein Machinery gene
  M0696_RS12720 (M0696_12720) comER 2524800..2525621 (+) 822 WP_222143197.1 late competence protein ComER -
  M0696_RS12725 (M0696_12725) - 2525687..2526430 (-) 744 WP_222143198.1 class I SAM-dependent methyltransferase -
  M0696_RS12730 (M0696_12730) rsfS 2526427..2526783 (-) 357 WP_166850602.1 ribosome silencing factor -
  M0696_RS12735 (M0696_12735) yqeK 2526801..2527361 (-) 561 WP_166850599.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M0696_RS12740 (M0696_12740) - 2527351..2527920 (-) 570 WP_166850596.1 nicotinate-nucleotide adenylyltransferase -
  M0696_RS12745 (M0696_12745) yhbY 2527932..2528222 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  M0696_RS12750 (M0696_12750) aroE 2528216..2529058 (-) 843 WP_222143199.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21905.69 Da        Isoelectric Point: 4.7586

>NTDB_id=681969 M0696_RS12715 WP_222143196.1 2524099..2524716(-) (comEA) [Bacillus rugosus strain A78.1]
MNWLNQHKKAIVLAASAAVFIAVIVFLASGKNKEPVKHAVSTETENTEVKQEEVKDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEMQVNLAELLQDGIVVYIPKRGEESAVQQAAGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=681969 M0696_RS12715 WP_222143196.1 2524099..2524716(-) (comEA) [Bacillus rugosus strain A78.1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCTGCTTCTGCGGCTGTTTTCATAGCGGTTATTGTCTTCCT
GGCCAGCGGAAAAAACAAAGAGCCGGTGAAGCACGCTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGAAG
TCAAAGACGAGTCAAACGAAACAATTGTGATTGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGATAGAGTGTCTCAGGCAATTGAGAAAGCAGGCGGAACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGATAGTAGTGTACATCCCGAAAAGGGGGGAGGAGTCAGCGGTGCAGCAAGCAGCCGGAGGCGCTA
TCCAGAGCGATGGAGGGAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAGACAATTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCCTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

89.756

100

0.898
  comEA Staphylococcus aureus N315

36.364

100

0.39
  comEA Staphylococcus aureus MW2

35.909

100

0.385