Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   M1F50_RS07910 Genome accession   NZ_CP096528
Coordinates   1636083..1636580 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain RIVM_M084986     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1631083..1641580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F50_RS07885 (M1F50_07865) gcvPB 1631245..1632717 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  M1F50_RS07890 (M1F50_07870) gcvPA 1632710..1634056 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  M1F50_RS07895 (M1F50_07875) gcvT 1634076..1635167 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  M1F50_RS07900 (M1F50_07880) - 1635326..1635850 (-) 525 WP_001015120.1 shikimate kinase -
  M1F50_RS07905 (M1F50_07885) - 1635840..1635986 (-) 147 WP_001789879.1 hypothetical protein -
  M1F50_RS07910 (M1F50_07890) comGF 1636083..1636580 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F50_RS07915 (M1F50_07895) comGE 1636498..1636797 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F50_RS07920 (M1F50_07900) comGD 1636784..1637230 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F50_RS07925 (M1F50_07905) comGC 1637208..1637519 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F50_RS07930 (M1F50_07910) comGB 1637533..1638603 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F50_RS07935 (M1F50_07915) comGA 1638575..1639549 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F50_RS07940 (M1F50_07920) - 1639601..1640224 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  M1F50_RS07945 (M1F50_07925) - 1640221..1640550 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F50_RS07950 (M1F50_07930) - 1640550..1641536 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=681515 M1F50_RS07910 WP_001825340.1 1636083..1636580(-) (comGF) [Staphylococcus aureus strain RIVM_M084986]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=681515 M1F50_RS07910 WP_001825340.1 1636083..1636580(-) (comGF) [Staphylococcus aureus strain RIVM_M084986]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97