Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PALA25_RS26320 Genome accession   NZ_CP109834
Coordinates   5700336..5700959 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain PALA25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5695336..5705959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA25_RS26300 (PALA25_05244) aroB 5695943..5697049 (-) 1107 WP_016253983.1 3-dehydroquinate synthase -
  PALA25_RS26305 (PALA25_05245) aroK 5697099..5697617 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA25_RS26310 (PALA25_05246) pilQ 5697629..5699761 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  PALA25_RS26315 (PALA25_05247) comL/pilP 5699815..5700339 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PALA25_RS26320 (PALA25_05248) pilO 5700336..5700959 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  PALA25_RS26325 (PALA25_05249) pilN 5700956..5701552 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  PALA25_RS26330 (PALA25_05250) pilM 5701552..5702616 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PALA25_RS26335 (PALA25_05251) - 5702801..5705272 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=680071 PALA25_RS26320 WP_003116135.1 5700336..5700959(-) (pilO) [Pseudomonas aeruginosa strain PALA25]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=680071 PALA25_RS26320 WP_003116135.1 5700336..5700959(-) (pilO) [Pseudomonas aeruginosa strain PALA25]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAAGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396