Detailed information NTG0678903

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MYA83_RS04225
• Genome accession: NZ_CP095871
• Coordinates: 958429..959295 (-)
• Length: 288 a.a.
• NCBI ID: WP_104995485.1
• Uniprot ID: A0A2L1JF49
• Organism: Pseudomonas palleroniana strain B-BH16-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 953429..964295

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MYA83_RS04190 (MYA83_04175)	-	954265..955563 (-)	1299	WP_419794591.1	NAD(P)/FAD-dependent oxidoreductase	-
MYA83_RS04195 (MYA83_04180)	-	955624..955797 (-)	174	WP_060754865.1	DUF3094 family protein	-
MYA83_RS04200 (MYA83_04185)	-	955887..956516 (-)	630	WP_053254334.1	DUF1780 domain-containing protein	-
MYA83_RS04205 (MYA83_04190)	-	956645..957334 (+)	690	WP_161548296.1	energy-coupling factor ABC transporter permease	-
MYA83_RS04210 (MYA83_04195)	-	957400..957615 (+)	216	WP_003171680.1	hypothetical protein	-
MYA83_RS04215 (MYA83_04200)	yacG	957612..957812 (-)	201	WP_060754867.1	DNA gyrase inhibitor YacG	-
MYA83_RS04220 (MYA83_04205)	coaE	957809..958432 (-)	624	WP_060754868.1	dephospho-CoA kinase	-
MYA83_RS04225 (MYA83_04210)	pilD	958429..959295 (-)	867	WP_104995485.1	prepilin peptidase	Machinery gene
MYA83_RS04230 (MYA83_04215)	pilC	959292..960500 (-)	1209	WP_104995486.1	type II secretion system F family protein	Machinery gene
MYA83_RS04235 (MYA83_04220)	-	960704..961117 (+)	414	WP_060754871.1	pilin	-
MYA83_RS04245 (MYA83_04230)	-	961418..961720 (+)	303	WP_060754872.1	DUF6388 family protein	-
MYA83_RS04250 (MYA83_04235)	nadC	961865..962713 (-)	849	WP_090366842.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31669.83 Da
• Isoelectric Point: 8.3879

>NTDB_id=678903 MYA83_RS04225 WP_104995485.1 958429..959295(-) (pilD) [Pseudomonas palleroniana strain B-BH16-1]
MSRLLTELPWAFVATALVLGLIVGSFLNVLVWRLPKMLEREWRAQANELLGLPTAPVGPAYNLMRPNSCCPRCNQPISPW
QNIPLLSYVMLRGRCAHCREPISTRYPLTELACALISAVTAWHFGFGWQAGAVMLLSWGLLAMSLIDAEHQLLPDVLVLP
LLWLGLILNSTGLLATLPDALWGAVTGYMSLWTVFWLFKLITGKDGMGHGDFKLLALLGAWGGWQILPMTLLLASLLGVS
AGLMLMRLRKSDASTPMPFGPCLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=678903 MYA83_RS04225 WP_104995485.1 958429..959295(-) (pilD) [Pseudomonas palleroniana strain B-BH16-1]
TTGAGCCGGCTGCTCACTGAGCTTCCCTGGGCCTTCGTTGCAACGGCGCTGGTACTCGGCCTGATCGTCGGCAGCTTCCT
CAATGTGCTGGTGTGGCGCCTGCCGAAGATGCTTGAACGGGAATGGCGTGCCCAGGCCAACGAACTCCTCGGACTGCCAA
CTGCGCCTGTCGGGCCTGCCTATAACCTGATGCGCCCCAATTCCTGCTGCCCGCGGTGCAATCAACCGATTAGCCCCTGG
CAAAATATCCCGCTGCTCAGCTACGTGATGCTCAGGGGGCGCTGCGCCCATTGTCGCGAGCCCATCAGCACCCGCTACCC
GTTGACTGAACTGGCCTGCGCACTCATTTCGGCCGTCACTGCCTGGCACTTCGGTTTCGGCTGGCAAGCAGGCGCAGTGA
TGCTGCTGAGTTGGGGCTTACTGGCCATGAGCCTGATCGATGCAGAGCATCAACTGCTGCCGGACGTGCTCGTGCTGCCA
CTGCTATGGCTGGGGCTGATCCTCAATAGCACTGGCCTGCTGGCGACACTGCCCGATGCGCTGTGGGGCGCGGTCACGGG
CTATATGAGCCTGTGGACGGTGTTCTGGCTGTTCAAGTTGATCACCGGCAAGGACGGCATGGGCCATGGCGACTTCAAAT
TACTGGCCCTGCTCGGCGCCTGGGGGGGTTGGCAGATTCTGCCGATGACGTTGCTGTTGGCGTCGCTGCTGGGGGTGTCT
GCCGGGCTGATGCTGATGCGCTTGCGCAAGTCCGACGCATCGACGCCGATGCCATTTGGTCCTTGCCTGGCGATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

3D structure

• AlphaFold DB: A0A2L1JF49

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.113	98.611	0.514
pilD	Vibrio campbellii strain DS40M4	47.735	99.653	0.476
pilD	Acinetobacter nosocomialis M2	46.479	98.611	0.458
pilD	Acinetobacter baumannii D1279779	46.479	98.611	0.458
pilD	Neisseria gonorrhoeae MS11	48.148	93.75	0.451