Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MRK30_RS18140 Genome accession   NZ_CP095357
Coordinates   3698638..3699498 (+) Length   286 a.a.
NCBI ID   WP_032070754.1    Uniprot ID   A0AAE9M8Y4
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3693638..3704498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS18125 (MRK30_18155) - 3694529..3695619 (+) 1091 WP_085947913.1 IS4-like element ISAba1 family transposase -
  MRK30_RS18130 (MRK30_18160) pilB 3695673..3697385 (+) 1713 WP_032070753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRK30_RS18135 (MRK30_18165) pilC 3697412..3698638 (+) 1227 WP_126117775.1 type II secretion system F family protein Machinery gene
  MRK30_RS18140 (MRK30_18170) pilD 3698638..3699498 (+) 861 WP_032070754.1 A24 family peptidase Machinery gene
  MRK30_RS18145 (MRK30_18175) coaE 3699500..3700096 (+) 597 WP_032070755.1 dephospho-CoA kinase -
  MRK30_RS18150 (MRK30_18180) - 3700093..3701007 (-) 915 WP_032070756.1 DMT family transporter -
  MRK30_RS18155 (MRK30_18185) rlmB 3701043..3701792 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MRK30_RS18160 (MRK30_18190) - 3701895..3702221 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  MRK30_RS18165 (MRK30_18195) - 3702298..3703608 (-) 1311 WP_032063688.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32128.61 Da        Isoelectric Point: 6.4261

>NTDB_id=676499 MRK30_RS18140 WP_032070754.1 3698638..3699498(+) (pilD) [Acinetobacter pittii strain 19MO01SH04]
MQEIIAYFIQNLTALYIAVALLSLCIGSFLNVVIYRTPKMMEQDWQQECQILLNPELPIIEHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLMLRGKCGDCQHPISIRYPAIELLTMLCSLVVVMVFGPTLQMLWGLVLTWILIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=676499 MRK30_RS18140 WP_032070754.1 3698638..3699498(+) (pilD) [Acinetobacter pittii strain 19MO01SH04]
ATGCAAGAAATTATTGCTTATTTTATTCAAAACTTAACTGCACTTTATATTGCAGTCGCACTATTGAGCCTATGTATTGG
TAGCTTTTTGAATGTGGTAATTTATCGCACCCCCAAAATGATGGAACAAGATTGGCAGCAGGAATGTCAAATATTGCTTA
ATCCCGAACTACCCATCATTGAGCATGAAAAGCTGACATTAAGTAAACCTGCTTCATCATGTCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCAGTTATAAGCTGGTTAATGTTAAGAGGAAAATGTGGCGATTGCCAACATCCTATTAG
CATTCGTTATCCAGCGATTGAATTACTCACGATGCTATGTTCATTAGTAGTCGTCATGGTTTTTGGTCCAACCCTACAAA
TGCTTTGGGGGCTAGTGCTCACTTGGATTTTGATTGCTTTAACATTTATAGACTTTGATACTCAGCTACTACCTGACCGT
TTCACCCTACCTTTGGCTGCTCTCGGCTTAGGTATTAATACCTTTAATATTTACACCTCGCCTAACTCAGCTATTTGGGG
ATATTTAATCGGTTTTTTATGCCTTTGGATTGTGTATTACTTATTTAAAGTGATTACAGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCCGCATTAGGCGCGTGGATGGGGCCATTGATGCTGCCATTAATTGTGCTATTGTCATCTTTA
CTAGGTGCAATTATTGGCATCATTTTACTAAAATTACGTAATGATAATCAGCCCTTTGCGTTTGGGCCATATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGACCAAATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

95.804

100

0.958
  pilD Acinetobacter nosocomialis M2

95.455

100

0.955
  pilD Vibrio campbellii strain DS40M4

53.309

95.105

0.507
  pilD Vibrio cholerae strain A1552

53.309

95.105

0.507
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437