Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MRK30_RS01575 Genome accession   NZ_CP095357
Coordinates   324877..325656 (+) Length   259 a.a.
NCBI ID   WP_032057248.1    Uniprot ID   A0A009QPU8
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319877..330656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS01560 (MRK30_01565) ponA 320458..323019 (-) 2562 WP_032057246.1 penicillin-binding protein PBP1a -
  MRK30_RS01565 (MRK30_01570) pilM 323181..324239 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  MRK30_RS01570 (MRK30_01575) pilN 324239..324880 (+) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  MRK30_RS01575 (MRK30_01580) pilO 324877..325656 (+) 780 WP_032057248.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRK30_RS01580 (MRK30_01585) pilP 325668..326195 (+) 528 WP_005067086.1 pilus assembly protein PilP Machinery gene
  MRK30_RS01585 (MRK30_01590) pilQ 326258..328423 (+) 2166 WP_032063183.1 type IV pilus secretin PilQ Machinery gene
  MRK30_RS01590 (MRK30_01595) aroK 328435..329004 (+) 570 WP_002116987.1 shikimate kinase AroK -
  MRK30_RS01595 (MRK30_01600) aroB 329033..330115 (+) 1083 WP_002116776.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29449.53 Da        Isoelectric Point: 5.7456

>NTDB_id=676463 MRK30_RS01575 WP_032057248.1 324877..325656(+) (pilO) [Acinetobacter pittii strain 19MO01SH04]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGRSDIPVLNYSIKAKTYRYMGAVENQNNSDPQVINGESQI
NASDSRNAHIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=676463 MRK30_RS01575 WP_032057248.1 324877..325656(+) (pilO) [Acinetobacter pittii strain 19MO01SH04]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAATTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAGATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCGCGGTTGAAAATCAAAATAATAGTGATCCTCAAGTGATAAATGGTGAAAGTCAGATA
AATGCCTCCGATAGCCGTAATGCACATATTCCTTCTATAAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009QPU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.286

86.486

0.772
  comO Acinetobacter baylyi ADP1

71.552

89.575

0.641