Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MRK30_RS01585 Genome accession   NZ_CP095357
Coordinates   326258..328423 (+) Length   721 a.a.
NCBI ID   WP_032063183.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321258..333423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS01565 (MRK30_01570) pilM 323181..324239 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  MRK30_RS01570 (MRK30_01575) pilN 324239..324880 (+) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  MRK30_RS01575 (MRK30_01580) pilO 324877..325656 (+) 780 WP_032057248.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRK30_RS01580 (MRK30_01585) pilP 325668..326195 (+) 528 WP_005067086.1 pilus assembly protein PilP Machinery gene
  MRK30_RS01585 (MRK30_01590) pilQ 326258..328423 (+) 2166 WP_032063183.1 type IV pilus secretin PilQ Machinery gene
  MRK30_RS01590 (MRK30_01595) aroK 328435..329004 (+) 570 WP_002116987.1 shikimate kinase AroK -
  MRK30_RS01595 (MRK30_01600) aroB 329033..330115 (+) 1083 WP_002116776.1 3-dehydroquinate synthase -
  MRK30_RS01600 (MRK30_01605) - 330132..331001 (+) 870 WP_032057253.1 hypothetical protein -

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 78096.54 Da        Isoelectric Point: 6.5418

>NTDB_id=676465 MRK30_RS01585 WP_032063183.1 326258..328423(+) (pilQ) [Acinetobacter pittii strain 19MO01SH04]
MNHVFRQFSMGAVAIAIMQVASAQVSMTNIVPMQIAGQGTEIRVIFNGLPPQPQAYQLENPSRLILDFNKAQQNLIQSKV
AIGTNEASSVDVSSDDQRSRLTVNLKEAGAFTTRIEGNTFILKINSAQAVAKPVSASAMQTQGVSNIGFQRGSQGEGLIV
IDLLGGNTPVDVQQQGSKVVVRTLGSKIPTHLAKRLNVNDFATPVSTIESYNEKGNGVITIQSTGSYEYMAYQAENKLTI
SLKRPQDKNVTSLHKTPNYSGNKLSLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLRLKDVPWDQALDIILKTKNL
DKRRNGNVIWIAPVAELIKAEEEEAKAVAQSVKLAPLQTEYIQLKYAQAQDIMSLITQGSNNSNGMQRTSDGGNSTSTNL
NTGVDSLGNNVGSLLSPRGTITQDDRTNTLIINDTAQSIDQIRKMIDLLDVQVKQVMVEARIVRASTSFTKELGVKWGIL
SQGITNNNNLLVGGSETTLWNLREPKKDETTGGYKYTIERPDNLNVDLGVTNPAGSIAFGLISMSDFMLDLELSALQADG
YGEVISTPKVMTADKQPAKVATGQQVPYQTTTNSAAGATASTSFKDALLSLDVTPSITPDGKIQMKLDISKDSVSGAAPN
GELILNKNNIKTNVLVNNGETVILGGVFEQTTLNSQTKVPFFGDLPGIGRLFRKDQKSDDKQELLIFVTPRIVNDTLVRN
H

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=676465 MRK30_RS01585 WP_032063183.1 326258..328423(+) (pilQ) [Acinetobacter pittii strain 19MO01SH04]
ATGAATCACGTATTCCGTCAGTTTTCTATGGGAGCTGTGGCGATTGCAATTATGCAGGTTGCATCTGCACAGGTCAGCAT
GACTAATATTGTACCGATGCAAATAGCGGGTCAGGGAACAGAAATTCGAGTGATATTTAATGGCTTGCCACCGCAACCAC
AGGCCTATCAGCTTGAGAATCCTTCACGTTTGATTTTAGATTTTAATAAAGCGCAGCAGAATTTAATACAGTCAAAAGTA
GCGATTGGAACGAATGAAGCGAGTTCGGTCGATGTAAGCTCCGATGATCAGCGTTCACGTTTAACTGTAAATTTGAAAGA
GGCAGGTGCTTTTACTACTCGTATTGAAGGCAACACTTTTATTTTAAAGATTAATTCTGCGCAAGCTGTAGCTAAACCTG
TATCAGCAAGTGCAATGCAAACCCAAGGTGTTTCAAACATCGGCTTCCAACGCGGTAGTCAAGGGGAAGGCCTTATCGTT
ATTGACCTGTTGGGAGGTAATACCCCTGTTGATGTGCAACAACAAGGTAGCAAAGTTGTAGTAAGAACGTTAGGCTCTAA
AATTCCTACACATCTAGCTAAACGCTTAAATGTAAATGATTTTGCGACGCCTGTTTCGACCATTGAATCTTATAATGAAA
AAGGCAATGGCGTTATTACGATTCAATCTACTGGTAGCTATGAATATATGGCTTATCAGGCAGAAAATAAATTAACCATT
AGCCTCAAGCGTCCGCAGGACAAAAATGTAACCTCTTTACATAAGACGCCTAATTACTCTGGTAATAAGCTTTCTCTCGA
TTTTCAAGATATTGAAGTTCGTCGCGTTTTACAGTTACTTGCTGATTTTACTGGCATTAATATGGTCGCGGCTGATAGTG
TGCAAGGTAATATCACTTTGCGTTTAAAAGATGTACCATGGGACCAAGCACTTGATATTATTTTAAAAACTAAAAATTTA
GATAAACGCAGAAATGGAAATGTAATTTGGATTGCTCCAGTTGCGGAACTGATCAAGGCTGAAGAAGAAGAAGCAAAAGC
TGTTGCACAGAGCGTGAAATTAGCCCCATTACAAACTGAGTATATTCAACTTAAATATGCGCAAGCTCAGGATATTATGA
GTTTAATTACTCAAGGCTCAAATAATAGCAATGGTATGCAGCGCACATCTGATGGTGGAAATTCTACATCTACTAATTTA
AATACTGGTGTTGATAGTTTAGGTAATAATGTAGGAAGTTTATTGAGTCCCCGTGGAACAATTACTCAAGATGATCGTAC
CAATACCTTAATTATTAATGATACGGCTCAGTCGATTGATCAAATTCGTAAAATGATTGATCTGTTGGATGTACAAGTTA
AACAGGTTATGGTTGAGGCGCGAATTGTAAGAGCATCTACTTCTTTTACCAAAGAGCTCGGTGTTAAATGGGGTATATTA
TCGCAAGGGATCACTAACAATAATAATTTGTTAGTTGGTGGTAGTGAAACGACCTTGTGGAATTTACGAGAGCCCAAAAA
GGATGAGACTACGGGTGGTTATAAATACACGATTGAACGTCCAGATAACTTAAATGTCGATTTAGGTGTAACCAATCCGG
CTGGGAGTATTGCCTTTGGCTTGATTAGTATGTCTGATTTTATGCTTGATCTTGAACTTTCTGCATTACAAGCTGATGGC
TATGGTGAGGTTATTTCTACCCCTAAAGTAATGACTGCAGATAAACAGCCTGCAAAAGTTGCAACAGGTCAACAGGTCCC
ATATCAAACTACAACGAATTCTGCTGCTGGTGCTACAGCAAGTACCTCTTTTAAAGATGCACTATTAAGCCTAGATGTTA
CCCCGAGTATTACGCCTGATGGTAAAATTCAAATGAAATTGGATATTTCAAAGGATTCGGTTTCGGGTGCGGCTCCTAAT
GGTGAATTGATCCTAAATAAAAATAATATCAAGACAAATGTGTTGGTGAATAATGGTGAGACAGTCATTTTAGGAGGGGT
GTTTGAGCAGACAACCCTTAACTCTCAAACCAAAGTTCCTTTCTTTGGGGATTTACCGGGAATTGGACGCCTATTTAGGA
AAGATCAGAAATCTGATGATAAGCAAGAGTTACTGATTTTCGTTACGCCACGAATTGTTAATGACACTTTGGTGAGAAAT
CATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(291-333)

AMIN domain (AMIN)

(40-118)

Bacterial type II and III secretion system protein (Secretin)

(555-713)

Bacterial type II/III secretion system short domain (Secretin_N)

(360-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

91.956

100

0.92
  pilQ Acinetobacter baumannii strain A118

91.817

100

0.918
  comQ Acinetobacter baylyi ADP1

74.965

100

0.752
  pilQ Pseudomonas aeruginosa PAK

39.86

99.168

0.395