Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MRO11_RS12530 Genome accession   NZ_CP095336
Coordinates   2745421..2746011 (-) Length   196 a.a.
NCBI ID   WP_154169997.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 19GA03SH07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2740421..2751011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO11_RS12510 (MRO11_12465) aroB 2741345..2742430 (-) 1086 WP_004394485.1 3-dehydroquinate synthase -
  MRO11_RS12515 (MRO11_12470) aroK 2742459..2742977 (-) 519 WP_040903956.1 shikimate kinase AroK -
  MRO11_RS12520 (MRO11_12475) pilQ 2743164..2744885 (-) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  MRO11_RS12525 (MRO11_12480) pilP 2744910..2745431 (-) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  MRO11_RS12530 (MRO11_12485) pilO 2745421..2746011 (-) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  MRO11_RS12535 (MRO11_12490) pilN 2746004..2746597 (-) 594 WP_161426721.1 PilN domain-containing protein Machinery gene
  MRO11_RS12540 (MRO11_12495) pilM 2746587..2747594 (-) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  MRO11_RS12545 (MRO11_12500) - 2747736..2750216 (+) 2481 WP_110167722.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22373.53 Da        Isoelectric Point: 4.5506

>NTDB_id=676446 MRO11_RS12530 WP_154169997.1 2745421..2746011(-) (pilO) [Vibrio metschnikovii strain 19GA03SH07]
MANFRDLDIDELLEWPLNAQLTVIVLVMVVIQVIGYGMYLSPKQEALEGLNQQEQSLKSLVRIKANKVAALPQLQTQLDE
LAERYDFLLRQLPAQQELASLLASVNELGLEHGLTFTRIDWGEKQPQAFLYRLPLNIELTGSYHNIGDFSQAIATLPRII
NVDDVDWQRVSQESSTLHFRVRAYTYQFKAEAGDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=676446 MRO11_RS12530 WP_154169997.1 2745421..2746011(-) (pilO) [Vibrio metschnikovii strain 19GA03SH07]
ATGGCTAATTTTCGTGATTTAGATATCGATGAATTGCTTGAATGGCCGCTCAACGCACAACTGACGGTGATTGTGTTGGT
CATGGTAGTGATTCAAGTCATCGGCTATGGCATGTATTTATCACCTAAACAAGAGGCACTCGAAGGGCTCAATCAGCAAG
AGCAGAGTCTTAAATCATTAGTACGCATTAAGGCCAATAAAGTCGCGGCATTACCTCAGTTGCAAACTCAATTGGATGAG
TTAGCTGAGCGTTATGATTTTTTATTGCGCCAACTCCCAGCACAGCAAGAGCTGGCCAGTTTGCTTGCTTCCGTCAACGA
GCTGGGATTAGAACATGGGTTAACCTTTACTCGAATTGATTGGGGAGAAAAGCAACCGCAGGCATTTTTGTATCGCTTAC
CATTGAATATTGAACTGACGGGAAGTTACCACAACATTGGCGATTTTTCTCAGGCGATAGCCACGCTACCAAGAATCATC
AATGTTGATGATGTGGATTGGCAAAGAGTCAGTCAAGAGAGCAGTACCTTACATTTCAGAGTACGGGCTTACACCTATCA
ATTTAAAGCGGAGGCAGGCGATGAGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

72.449

100

0.724
  pilO Vibrio campbellii strain DS40M4

67.725

96.429

0.653