Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MRN59_RS04400 Genome accession   NZ_CP095314
Coordinates   790605..791576 (+) Length   323 a.a.
NCBI ID   WP_133445016.1    Uniprot ID   -
Organism   Macrococcoides caseolyticum strain 19MO06SA05     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 785605..796576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRN59_RS04360 (MRN59_04350) - 785694..786248 (+) 555 WP_354680532.1 5-formyltetrahydrofolate cyclo-ligase -
  MRN59_RS04365 (MRN59_04355) - 786224..787672 (+) 1449 WP_354680533.1 rhomboid family intramembrane serine protease -
  MRN59_RS04370 (MRN59_04360) - 787677..787868 (+) 192 WP_133445012.1 YqgQ family protein -
  MRN59_RS04375 (MRN59_04365) - 787865..788833 (+) 969 WP_354680534.1 ROK family glucokinase -
  MRN59_RS04380 (MRN59_04370) - 788833..789144 (+) 312 WP_133445014.1 MTH1187 family thiamine-binding protein -
  MRN59_RS04385 (MRN59_04375) - 789169..789408 (-) 240 WP_041636024.1 DUF2759 domain-containing protein -
  MRN59_RS04390 (MRN59_04380) - 789498..790118 (+) 621 WP_133445015.1 MBL fold metallo-hydrolase -
  MRN59_RS04395 (MRN59_04385) - 790145..790393 (-) 249 WP_041636023.1 DUF2626 domain-containing protein -
  MRN59_RS04400 (MRN59_04390) comGA 790605..791576 (+) 972 WP_133445016.1 competence type IV pilus ATPase ComGA Machinery gene
  MRN59_RS04405 (MRN59_04395) comGB 791527..792600 (+) 1074 WP_133445017.1 competence type IV pilus assembly protein ComGB -
  MRN59_RS04410 (MRN59_04400) comGC 792622..792945 (+) 324 WP_041636417.1 competence type IV pilus major pilin ComGC Machinery gene
  MRN59_RS04415 (MRN59_04405) - 792938..793363 (+) 426 WP_012657089.1 type II secretion system protein -
  MRN59_RS04420 (MRN59_04410) - 793350..793592 (+) 243 WP_133445018.1 hypothetical protein -
  MRN59_RS04425 (MRN59_04415) comGF 793537..793989 (+) 453 WP_133445019.1 competence type IV pilus minor pilin ComGF -
  MRN59_RS04430 (MRN59_04420) - 794150..794656 (+) 507 WP_101140318.1 shikimate kinase -
  MRN59_RS04435 (MRN59_04425) gcvT 794794..795879 (+) 1086 WP_101140329.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37169.81 Da        Isoelectric Point: 6.2403

>NTDB_id=676331 MRN59_RS04400 WP_133445016.1 790605..791576(+) (comGA) [Macrococcoides caseolyticum strain 19MO06SA05]
MEKLFNEIIEQAILQSASDIHFIPCDKNVSIKFRVQGDIEEYSDIDDILFKKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
DNLRYNIRASTLPRSLGDEACVLRIIRQSFIDEYQTDDQILFDQMKKSSGIIIISGPTGSGKSTLMYQLVHFAKDTLKRQ
VISIEDPVEQHLDGIIQVNVNEKAEITYQTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLIKKRDEKERILAYEFLTKKNIEKYLKNKHINYRTLADILKEMYETNQISQHEFEK
FDF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=676331 MRN59_RS04400 WP_133445016.1 790605..791576(+) (comGA) [Macrococcoides caseolyticum strain 19MO06SA05]
ATGGAGAAATTATTCAATGAAATAATAGAGCAGGCAATTTTACAAAGTGCATCAGATATACACTTCATTCCTTGTGATAA
AAATGTATCTATTAAATTTAGGGTACAAGGTGATATCGAAGAATATAGTGACATCGACGATATATTATTTAAAAAATTAC
TTTCATATATTAAATTTACAGCACACCTTGATGTATCAGAAAAGAATAAGGCTCAGAGTGGGATAATACATTTTAATCTG
GATAACTTACGATATAATATTCGCGCATCTACTTTACCTCGTTCATTAGGCGATGAAGCATGTGTATTGAGAATCATCAG
ACAAAGTTTTATAGATGAATATCAGACAGATGATCAGATATTGTTCGATCAGATGAAAAAATCAAGCGGTATAATTATTA
TTAGTGGGCCAACTGGAAGTGGTAAGAGTACATTAATGTATCAACTTGTACATTTTGCAAAGGACACATTGAAACGCCAA
GTAATTTCAATAGAAGATCCTGTGGAGCAGCATCTTGACGGTATCATACAAGTTAATGTTAACGAAAAAGCAGAAATAAC
ATATCAAACCGCAATTAAGGCAATCCTTAGATGTGATCCAGATATCATTATGCTAGGTGAAATAAGAGATTCGGTCGTAG
CACAGCAAGTTATCAATGCGGGGCTGAGTGGTCATTTAGTTTTAACGACATTACATGCGAATGATTGTATTGGCGCATTA
TTTCGGTTAAAAGAAATGGGAATTAATGCTGTTGATCTTTATCAAAGTATCAATTTGATAATCAATCAAAGACTGATAAA
AAAAAGAGATGAAAAGGAGCGTATTCTAGCCTATGAATTTCTGACAAAAAAGAATATTGAAAAATATTTAAAGAATAAGC
ACATAAACTATAGGACGTTGGCTGACATACTAAAGGAGATGTATGAAACAAATCAGATTTCACAACATGAATTTGAAAAA
TTTGATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.923

100

0.492
  comGA Staphylococcus aureus N315

48.923

100

0.492
  comGA Bacillus subtilis subsp. subtilis str. 168

34.857

100

0.378