Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUU70_RS11465 Genome accession   NZ_CP095093
Coordinates   2389540..2390610 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain D103     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2384540..2395610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU70_RS11415 (MUU70_11430) sipW 2384600..2385184 (-) 585 WP_367069085.1 signal peptidase I SipW -
  MUU70_RS11420 (MUU70_11435) tapA 2385156..2385827 (-) 672 WP_367069086.1 amyloid fiber anchoring/assembly protein TapA -
  MUU70_RS11425 (MUU70_11440) - 2386086..2386415 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  MUU70_RS11430 (MUU70_11445) - 2386455..2386634 (-) 180 WP_003153093.1 YqzE family protein -
  MUU70_RS11435 (MUU70_11450) comGG 2386691..2387068 (-) 378 WP_182069279.1 competence type IV pilus minor pilin ComGG Machinery gene
  MUU70_RS11440 (MUU70_11455) comGF 2387069..2387464 (-) 396 WP_077722593.1 competence type IV pilus minor pilin ComGF -
  MUU70_RS11445 (MUU70_11460) comGE 2387478..2387792 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  MUU70_RS11450 (MUU70_11465) comGD 2387776..2388213 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUU70_RS11455 (MUU70_11470) comGC 2388203..2388511 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  MUU70_RS11460 (MUU70_11475) comGB 2388516..2389553 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  MUU70_RS11465 (MUU70_11480) comGA 2389540..2390610 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  MUU70_RS11470 (MUU70_11485) - 2390802..2391752 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  MUU70_RS11475 (MUU70_11490) - 2391898..2393199 (+) 1302 WP_070082114.1 hemolysin family protein -
  MUU70_RS11480 (MUU70_11495) - 2393237..2394067 (-) 831 WP_367069087.1 STAS domain-containing protein -
  MUU70_RS11490 (MUU70_11505) - 2394273..2394653 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MUU70_RS11495 (MUU70_11510) - 2394890..2395135 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=675322 MUU70_RS11465 WP_003153083.1 2389540..2390610(-) (comGA) [Bacillus velezensis strain D103]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=675322 MUU70_RS11465 WP_003153083.1 2389540..2390610(-) (comGA) [Bacillus velezensis strain D103]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACAGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374