Detailed information NTG0673945

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: lgb_RS04235
• Genome accession: NZ_CP094882
• Coordinates: 839342..840598 (-)
• Length: 418 a.a.
• NCBI ID: WP_019293559.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain B1726
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 834342..845598

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
lgb_RS04200 (lgb_00833)	-	834504..834785 (-)	282	WP_227671623.1	hypothetical protein	-
lgb_RS04205 (lgb_00834)	ald	835095..836201 (+)	1107	WP_019293562.1	alanine dehydrogenase	-
lgb_RS04210 (lgb_00835)	-	836321..837280 (+)	960	WP_249663293.1	PhoH family protein	-
lgb_RS04215 (lgb_00836)	-	837282..837755 (+)	474	WP_019292774.1	NUDIX hydrolase	-
lgb_RS04220 (lgb_00837)	ybeY	837765..838253 (+)	489	WP_019293561.1	rRNA maturation RNase YbeY	-
lgb_RS04225 (lgb_00838)	-	838237..838698 (+)	462	WP_019292772.1	diacylglycerol kinase family protein	-
lgb_RS04230 (lgb_00839)	-	838701..839345 (-)	645	WP_019293560.1	ComF family protein	-
lgb_RS04235 (lgb_00840)	comFA	839342..840598 (-)	1257	WP_019293559.1	DEAD/DEAH box helicase	Machinery gene
lgb_RS04240 (lgb_00841)	-	840653..841276 (+)	624	WP_019293558.1	YigZ family protein	-
lgb_RS04245 (lgb_00842)	-	841289..842311 (+)	1023	WP_019293557.1	DUF475 domain-containing protein	-
lgb_RS04250 (lgb_00843)	-	842346..843368 (-)	1023	WP_019293556.1	alpha/beta hydrolase	-
lgb_RS04255 (lgb_00844)	-	843686..844465 (+)	780	WP_019292766.1	formate/nitrite transporter family protein	-
lgb_RS04260 (lgb_00845)	glyQ	844479..845447 (+)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47952.84 Da
• Isoelectric Point: 10.1309

>NTDB_id=673945 lgb_RS04235 WP_019293559.1 839342..840598(-) (comFA) [Lactococcus petauri strain B1726]
MNELYGRLLLQKELSKIPDKAILFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTPPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLSQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=673945 lgb_RS04235 WP_019293559.1 839342..840598(-) (comFA) [Lactococcus petauri strain B1726]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAATACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGCGGTAAAAAAAGTAAGAAAAAAGAGGTCTTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCACCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACACCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAGCCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGATGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (93-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.642	95.455	0.541
comFA/cflA	Streptococcus mitis SK321	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae D39	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae R6	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae TIGR4	53.535	94.737	0.507
comFA/cflA	Streptococcus mitis NCTC 12261	52.02	94.737	0.493
comFA	Bacillus subtilis subsp. subtilis str. 168	37.671	100	0.395
comFA	Latilactobacillus sakei subsp. sakei 23K	39.295	94.976	0.373