Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUA23_RS07090 Genome accession   NZ_CP094816
Coordinates   1395076..1395978 (+) Length   300 a.a.
NCBI ID   WP_078355838.1    Uniprot ID   A0AAI8DHB9
Organism   Mammaliicoccus sciuri strain IVB6231     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1390076..1400978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA23_RS07050 (MUA23_07040) - 1390161..1390721 (+) 561 WP_078355842.1 5-formyltetrahydrofolate cyclo-ligase -
  MUA23_RS07055 (MUA23_07045) - 1390714..1392162 (+) 1449 WP_107603465.1 rhomboid family intramembrane serine protease -
  MUA23_RS07060 (MUA23_07050) - 1392167..1392358 (+) 192 WP_078099923.1 YqgQ family protein -
  MUA23_RS07065 (MUA23_07055) - 1392355..1393326 (+) 972 WP_048539719.1 ROK family glucokinase -
  MUA23_RS07070 (MUA23_07060) - 1393326..1393640 (+) 315 WP_078355840.1 MTH1187 family thiamine-binding protein -
  MUA23_RS07075 (MUA23_07065) - 1393670..1393909 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  MUA23_RS07080 (MUA23_07070) - 1394007..1394639 (+) 633 WP_078355839.1 MBL fold metallo-hydrolase -
  MUA23_RS07085 (MUA23_07075) - 1394674..1394922 (-) 249 WP_025904960.1 DUF2626 family protein -
  MUA23_RS07090 (MUA23_07080) comGA 1395076..1395978 (+) 903 WP_078355838.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA23_RS07095 (MUA23_07085) comGB 1395968..1397008 (+) 1041 WP_088592324.1 competence type IV pilus assembly protein ComGB -
  MUA23_RS07100 (MUA23_07090) comGC 1397023..1397340 (+) 318 WP_262657651.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA23_RS07105 (MUA23_07095) - 1397369..1397767 (+) 399 WP_262657680.1 prepilin-type cleavage/methylation domain-containing protein -
  MUA23_RS07110 (MUA23_07100) comGE 1397751..1398032 (+) 282 WP_048539723.1 competence type IV pilus minor pilin ComGE -
  MUA23_RS07115 (MUA23_07105) - 1398007..1398441 (+) 435 WP_078355835.1 competence type IV pilus minor pilin ComGF -
  MUA23_RS07120 (MUA23_07110) - 1398676..1399164 (+) 489 WP_048539725.1 shikimate kinase -
  MUA23_RS07125 (MUA23_07115) gcvT 1399357..1400445 (+) 1089 WP_078355834.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34536.56 Da        Isoelectric Point: 6.4992

>NTDB_id=673198 MUA23_RS07090 WP_078355838.1 1395076..1395978(+) (comGA) [Mammaliicoccus sciuri strain IVB6231]
MEKLFKSIINDAVNSNASDIHFVPLNDDKVFVQMRINGDNIPYDLDLDFKLYSRLLIYMKFIAHLDVSERNKAQSGQYLY
EHDIHYYLRISTIPISLGIESCSIRITPQRFNHSQVKSEHQSYNVLQNRMYQSEGLFLFTGPTGSGKTTLMYELMYALKR
EKNKHIITIEDPVEQTLNGIVQVSVNEKANITYQQSFKAILRCDPDVIMIGEIRDDVTAKQVIQASLSGHLVLSTMHASD
TIGAIHRLIELGVTVEQIKQGISCITNQRLVKKNEETRILNMSYIFKDDIIKYMENDYET

Nucleotide


Download         Length: 903 bp        

>NTDB_id=673198 MUA23_RS07090 WP_078355838.1 1395076..1395978(+) (comGA) [Mammaliicoccus sciuri strain IVB6231]
ATGGAGAAATTATTTAAGTCAATTATTAACGATGCTGTCAATTCAAATGCGAGTGATATTCACTTTGTACCTTTAAATGA
TGATAAAGTCTTTGTTCAAATGAGGATAAATGGCGATAATATACCTTATGATTTAGATCTAGACTTTAAATTGTATAGTA
GGCTACTCATTTATATGAAATTTATCGCTCATCTTGATGTAAGTGAACGTAATAAAGCACAAAGCGGTCAATATTTATAT
GAACATGATATTCATTACTACTTAAGAATTTCTACGATACCTATCAGTTTAGGAATAGAATCTTGTTCCATTAGAATTAC
CCCTCAACGTTTTAATCATTCACAAGTGAAATCAGAGCATCAATCATATAACGTTCTACAAAATAGAATGTATCAATCAG
AAGGGTTATTTCTATTTACGGGGCCAACAGGATCAGGTAAGACGACACTAATGTATGAATTGATGTACGCATTAAAAAGA
GAGAAGAATAAACATATTATTACGATTGAAGATCCTGTAGAACAAACATTAAATGGTATCGTTCAAGTTTCAGTTAATGA
AAAAGCTAACATAACTTATCAACAATCCTTTAAAGCTATTTTAAGATGTGATCCTGACGTTATTATGATTGGGGAGATAA
GAGATGATGTTACTGCTAAGCAAGTTATTCAGGCAAGTTTAAGTGGTCATTTAGTGCTTTCAACTATGCATGCTAGTGAT
ACAATCGGGGCAATCCATCGACTAATAGAACTAGGTGTAACAGTAGAACAAATTAAACAAGGTATTTCATGTATTACGAA
CCAACGTCTTGTGAAGAAAAATGAAGAAACAAGAATTTTAAATATGTCTTATATTTTTAAAGATGACATTATTAAATATA
TGGAGAATGATTATGAAACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

52.857

93.333

0.493
  comGA Staphylococcus aureus N315

52.857

93.333

0.493
  comGA Bacillus subtilis subsp. subtilis str. 168

40.433

92.333

0.373