Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUA83_RS08105 Genome accession   NZ_CP094763
Coordinates   1661426..1662400 (-) Length   324 a.a.
NCBI ID   WP_262528786.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IVB6221     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656426..1667400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA83_RS08065 (MUA83_08060) gcvT 1656927..1658018 (-) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  MUA83_RS08070 (MUA83_08065) - 1658177..1658701 (-) 525 WP_001015120.1 shikimate kinase -
  MUA83_RS08075 (MUA83_08070) - 1658691..1658837 (-) 147 WP_001789879.1 hypothetical protein -
  MUA83_RS08080 (MUA83_08075) comGF 1658934..1659431 (-) 498 WP_262528782.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUA83_RS08085 (MUA83_08080) comGE 1659349..1659648 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MUA83_RS08090 (MUA83_08085) comGD 1659635..1660081 (-) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUA83_RS08095 (MUA83_08090) comGC 1660059..1660370 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA83_RS08100 (MUA83_08095) comGB 1660384..1661454 (-) 1071 Protein_1593 competence type IV pilus assembly protein ComGB -
  MUA83_RS08105 (MUA83_08100) comGA 1661426..1662400 (-) 975 WP_262528786.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA83_RS08110 (MUA83_08105) - 1662452..1663075 (-) 624 WP_031872212.1 MBL fold metallo-hydrolase -
  MUA83_RS08115 (MUA83_08110) - 1663072..1663401 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MUA83_RS08120 (MUA83_08115) - 1663401..1664387 (-) 987 WP_000161315.1 ROK family glucokinase -
  MUA83_RS08125 (MUA83_08120) - 1664384..1664587 (-) 204 WP_000087561.1 YqgQ family protein -
  MUA83_RS08130 (MUA83_08125) - 1664568..1666031 (-) 1464 WP_044122531.1 rhomboid family intramembrane serine protease -
  MUA83_RS08135 (MUA83_08130) - 1666043..1666582 (-) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  MUA83_RS08140 (MUA83_08135) rpmG 1666791..1666940 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36911.98 Da        Isoelectric Point: 8.8516

>NTDB_id=672085 MUA83_RS08105 WP_262528786.1 1661426..1662400(-) (comGA) [Staphylococcus aureus strain IVB6221]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
IISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=672085 MUA83_RS08105 WP_262528786.1 1661426..1662400(-) (comGA) [Staphylococcus aureus strain IVB6221]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
ATAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997