Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MUA83_RS08080 Genome accession   NZ_CP094763
Coordinates   1658934..1659431 (-) Length   165 a.a.
NCBI ID   WP_262528782.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IVB6221     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653934..1664431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA83_RS08055 (MUA83_08050) gcvPB 1654096..1655568 (-) 1473 WP_000202180.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  MUA83_RS08060 (MUA83_08055) gcvPA 1655561..1656907 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  MUA83_RS08065 (MUA83_08060) gcvT 1656927..1658018 (-) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  MUA83_RS08070 (MUA83_08065) - 1658177..1658701 (-) 525 WP_001015120.1 shikimate kinase -
  MUA83_RS08075 (MUA83_08070) - 1658691..1658837 (-) 147 WP_001789879.1 hypothetical protein -
  MUA83_RS08080 (MUA83_08075) comGF 1658934..1659431 (-) 498 WP_262528782.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUA83_RS08085 (MUA83_08080) comGE 1659349..1659648 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MUA83_RS08090 (MUA83_08085) comGD 1659635..1660081 (-) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUA83_RS08095 (MUA83_08090) comGC 1660059..1660370 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA83_RS08100 (MUA83_08095) comGB 1660384..1661454 (-) 1071 Protein_1593 competence type IV pilus assembly protein ComGB -
  MUA83_RS08105 (MUA83_08100) comGA 1661426..1662400 (-) 975 WP_262528786.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA83_RS08110 (MUA83_08105) - 1662452..1663075 (-) 624 WP_031872212.1 MBL fold metallo-hydrolase -
  MUA83_RS08115 (MUA83_08110) - 1663072..1663401 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MUA83_RS08120 (MUA83_08115) - 1663401..1664387 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19194.93 Da        Isoelectric Point: 10.3044

>NTDB_id=672081 MUA83_RS08080 WP_262528782.1 1658934..1659431(-) (comGF) [Staphylococcus aureus strain IVB6221]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLKNNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=672081 MUA83_RS08080 WP_262528782.1 1658934..1659431(-) (comGF) [Staphylococcus aureus strain IVB6221]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAAAAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

96.364

100

0.964