Detailed information NTG0066934

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: GM3708_RS15745
• Genome accession: NZ_AP014815
• Coordinates: 3588142..3590901 (-)
• Length: 919 a.a.
• NCBI ID: WP_066348921.1
• Uniprot ID: A0A0D6AJ64
• Organism: Geminocystis sp. NIES-3708
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3583142..3595901

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GM3708_RS15720 (GM3708_3243)	-	3583623..3584198 (+)	576	WP_066348913.1	TerD family protein	-
GM3708_RS15725 (GM3708_3244)	-	3584351..3584944 (+)	594	WP_066348914.1	TerD family protein	-
GM3708_RS15730 (GM3708_3245)	-	3585035..3585784 (-)	750	WP_066348916.1	hypothetical protein	-
GM3708_RS15735 (GM3708_3246)	-	3586229..3587164 (-)	936	WP_066348917.1	NAD-dependent epimerase/dehydratase family protein	-
GM3708_RS15740 (GM3708_3247)	-	3587236..3588000 (-)	765	WP_082714161.1	MlaE family lipid ABC transporter permease subunit	-
GM3708_RS15745 (GM3708_3248)	pilJ	3588142..3590901 (-)	2760	WP_066348921.1	HAMP domain-containing methyl-accepting chemotaxis protein	Machinery gene
GM3708_RS15750 (GM3708_3249)	-	3590973..3591512 (-)	540	WP_066348922.1	chemotaxis protein CheW	-
GM3708_RS15755 (GM3708_3250)	pilH	3591832..3592197 (-)	366	WP_066348924.1	response regulator transcription factor	Machinery gene
GM3708_RS15760 (GM3708_3251)	murA	3592501..3593793 (-)	1293	WP_144439333.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
GM3708_RS15765 (GM3708_3252)	ruvX	3594017..3594433 (-)	417	WP_066348929.1	Holliday junction resolvase RuvX	-
GM3708_RS15770 (GM3708_3253)	-	3594495..3595388 (-)	894	WP_066348931.1	DUF4922 domain-containing protein	-

Sequence

Protein

• Length: 919 a.a.
• Molecular weight: 101424.03 Da
• Isoelectric Point: 3.9365

>NTDB_id=66934 GM3708_RS15745 WP_066348921.1 3588142..3590901(-) (pilJ) [Geminocystis sp. NIES-3708]
MVSETDYQQRYGQAQTAYLEGDLETANNITDSIIEDYPHDPSVLLLKGHIHLRQQKYEIAKATYEEVLSLTDHQDLVSLA
HQGLTQIQEEELDNFYPDEVETPDFEDDIEEIDDDEPNDNWTSSIQFDSIDWDVEDLEEEEIEDPTLQQNAPLNRDFSSP
PPKAKSSSPSDNDSFNPFDDDDDDDTAANVVIDFSKNDNQNSEFEPLADNLLEMDIDESDFDGFGNDENDFDANDLQETG
TPTFVVSSDEPELSDLEKMLTGGSELLGNFDEDFGDYSSELEPLTSAKKSTSFNTEIDEDDPLVPDGEDNFFFAPDELDD
IPDIGADELPVSSIFTKEQRADKSFNNIIDNNDSVFDDDFENEITGSFSFDTDRINLDIEDDEISVSSSLTDISSTPLSV
NVTPEVEIPQGNLAQYFDVPLFSKQLVHGGVTGLVTFLVVFFVSMLPATITPQTAEIKNTVTSAQKKENQKPSSTTQPKK
ETAKPTSKQSVSYPFGSRFFLSLLAGIASGGSTVAMGYLMSNHIKRYTNDLQNQFESVYQGNYEVKATVYSQDEFGTLAA
GFNQMAKMINTTTTEARKRAEETERAREDLQRQVIRLLDDVEGAARGDLTVQAEVTADVLGAVADAFNLTITNLRKIVKQ
VKQAAVQVNKASTDSEVFARNQSSDALRMAEELAVTLNSVQMMTDSIQRVAENAREAEEVARSSSVTALKGGDAVERTVA
GILQIRETVSETTRKVKRLAEASQQISTIVAVISQISSRTNLLALNASIQAARAGEAGRGFAIVAEEVRQLADRSAKSLQ
EIEQIVLQIQTETGSVMTAMEEGIQQVRDVTDRSEQAKRALEDIIQVSNRIDALVRSITADTDEQRENSRGVAKVMQAVE
LTAQATSQESQRVAGSLQNLVGIAKDLISSVERFKVEGK

Nucleotide

• Length: 2760 bp

>NTDB_id=66934 GM3708_RS15745 WP_066348921.1 3588142..3590901(-) (pilJ) [Geminocystis sp. NIES-3708]
ATGGTATCGGAAACTGATTATCAACAAAGATATGGACAAGCTCAAACAGCTTATCTGGAAGGTGATTTAGAGACTGCTAA
TAATATTACAGACTCAATTATCGAAGATTATCCTCATGATCCTAGTGTTTTATTACTTAAGGGTCATATTCATTTACGTC
AACAAAAATATGAAATTGCAAAAGCAACTTATGAAGAAGTATTGAGTTTAACAGATCATCAAGATTTAGTTAGCCTAGCT
CATCAAGGATTAACGCAAATACAAGAAGAAGAATTAGATAATTTTTATCCTGACGAAGTAGAAACTCCTGATTTTGAAGA
TGATATTGAGGAAATTGATGATGACGAGCCGAATGATAATTGGACTAGTAGCATTCAATTTGATAGTATTGACTGGGATG
TTGAAGATTTAGAAGAAGAAGAAATTGAAGATCCAACCTTACAACAAAATGCACCTTTAAATAGAGATTTTTCCTCTCCT
CCTCCGAAAGCAAAATCTTCTTCTCCTTCAGACAATGATAGTTTTAATCCCTTTGATGACGATGATGATGATGATACGGC
GGCGAATGTAGTTATTGATTTTTCCAAGAATGACAATCAAAATTCAGAATTTGAGCCTTTGGCGGATAATTTATTGGAGA
TGGATATTGACGAGTCTGATTTTGATGGTTTTGGGAATGATGAAAATGATTTTGATGCCAATGATTTACAAGAAACTGGT
ACTCCTACTTTTGTAGTTTCTTCTGATGAACCTGAGTTAAGTGATTTAGAAAAAATGTTAACTGGAGGTTCAGAATTATT
AGGTAATTTTGATGAAGATTTCGGCGATTATAGTTCTGAATTAGAGCCTTTAACTTCTGCGAAAAAATCTACTTCTTTTA
ATACTGAAATTGATGAAGATGATCCTTTAGTGCCAGACGGAGAAGATAATTTCTTTTTTGCTCCTGATGAATTGGACGAT
ATTCCTGATATTGGTGCTGATGAGTTACCTGTTTCTAGTATTTTTACTAAAGAGCAACGGGCTGATAAAAGTTTTAATAA
TATTATTGATAATAATGATAGTGTTTTTGATGATGATTTTGAAAATGAAATCACGGGTAGTTTTAGCTTTGATACTGATC
GCATAAATCTTGATATAGAAGATGACGAAATAAGTGTTTCTTCTTCTTTGACAGATATATCCAGTACTCCTTTGTCTGTA
AATGTTACCCCAGAAGTAGAAATTCCCCAAGGAAATCTTGCCCAATATTTTGATGTACCTTTATTTTCTAAACAATTAGT
TCACGGTGGCGTGACGGGTTTAGTAACTTTTTTGGTAGTGTTTTTCGTAAGTATGTTACCTGCAACCATTACGCCTCAAA
CTGCAGAAATTAAAAATACAGTCACGAGTGCACAAAAGAAAGAAAATCAGAAACCATCGTCAACCACACAGCCGAAAAAA
GAAACGGCAAAACCCACATCAAAACAATCTGTTAGCTATCCTTTTGGCAGTAGATTTTTCTTAAGTTTATTGGCAGGAAT
TGCCAGTGGAGGTTCAACGGTGGCAATGGGTTATTTGATGAGCAATCATATCAAACGTTATACTAATGACCTTCAAAATC
AATTTGAGTCGGTTTATCAAGGTAATTATGAAGTAAAAGCCACAGTTTACTCTCAAGATGAATTTGGTACTCTAGCCGCA
GGGTTTAACCAAATGGCGAAAATGATCAATACCACTACCACCGAAGCTCGAAAACGTGCGGAAGAAACAGAAAGAGCAAG
AGAAGATTTACAACGCCAAGTTATCCGCCTTCTTGATGACGTGGAGGGAGCTGCTAGAGGAGATTTAACGGTACAAGCAG
AAGTCACCGCAGACGTATTAGGTGCGGTAGCGGATGCTTTTAACCTAACCATTACTAACTTAAGAAAAATTGTTAAGCAG
GTAAAACAGGCTGCCGTACAAGTAAATAAAGCTAGTACAGATAGTGAGGTATTCGCCCGAAATCAATCCAGTGACGCTTT
AAGAATGGCTGAAGAACTAGCCGTAACTCTCAATTCCGTCCAAATGATGACGGACTCAATCCAAAGGGTAGCAGAAAACG
CTAGAGAAGCGGAAGAAGTTGCTAGATCTTCTAGTGTTACAGCTCTTAAAGGTGGTGATGCGGTAGAAAGAACTGTGGCA
GGTATTTTACAAATTAGGGAAACAGTATCAGAAACCACCAGAAAAGTTAAAAGATTAGCGGAAGCATCTCAACAAATTTC
GACCATTGTCGCTGTTATTTCTCAAATTTCTTCTCGTACTAACCTTCTAGCATTAAATGCTTCGATTCAGGCGGCACGGG
CGGGAGAAGCTGGTAGAGGATTTGCTATTGTTGCGGAAGAAGTGCGGCAGTTAGCTGATCGTTCTGCTAAATCTTTGCAA
GAAATTGAACAAATCGTATTACAAATTCAAACGGAAACAGGTTCGGTAATGACGGCGATGGAAGAAGGTATCCAACAGGT
AAGAGATGTAACTGATCGTTCTGAACAGGCAAAACGAGCTTTAGAAGATATTATTCAAGTATCTAACCGTATTGATGCTC
TAGTAAGATCAATTACTGCTGACACCGATGAACAACGAGAAAATTCAAGGGGAGTGGCAAAAGTAATGCAAGCGGTAGAA
TTAACTGCTCAGGCAACTTCTCAAGAATCACAGCGTGTTGCTGGGTCGCTCCAAAATCTTGTGGGTATTGCGAAGGATTT
AATTTCTTCTGTGGAACGTTTTAAAGTAGAAGGAAAATAA

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (521-570)

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (699-880)

3D structure

• AlphaFold DB: A0A0D6AJ64

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	48.36	100	0.497