Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GM3708_RS11405 Genome accession   NZ_AP014815
Coordinates   2578279..2580330 (-) Length   683 a.a.
NCBI ID   WP_066346987.1    Uniprot ID   A0A0D6AGM5
Organism   Geminocystis sp. NIES-3708     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2573279..2585330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3708_RS11380 (GM3708_2354) - 2574296..2575081 (-) 786 WP_066346974.1 D-alanyl-D-alanine carboxypeptidase family protein -
  GM3708_RS11385 (GM3708_2355) - 2575249..2576085 (+) 837 Protein_2310 methyltransferase domain-containing protein -
  GM3708_RS11390 (GM3708_2356) arsS 2576232..2577227 (+) 996 WP_066346984.1 arsenosugar biosynthesis radical SAM (seleno)protein ArsS -
  GM3708_RS11395 (GM3708_2357) - 2577281..2577982 (+) 702 WP_066346985.1 HAD family phosphatase -
  GM3708_RS11405 (GM3708_2358) pilB/pilB1 2578279..2580330 (-) 2052 WP_066346987.1 GspE/PulE family protein Machinery gene
  GM3708_RS11410 (GM3708_2359) grpE 2580829..2581575 (+) 747 WP_396229671.1 nucleotide exchange factor GrpE -
  GM3708_RS11415 (GM3708_2361) dnaK 2581660..2583678 (+) 2019 WP_066346995.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 683 a.a.        Molecular weight: 76520.73 Da        Isoelectric Point: 5.1577

>NTDB_id=66932 GM3708_RS11405 WP_066346987.1 2578279..2580330(-) (pilB/pilB1) [Geminocystis sp. NIES-3708]
MTQTPISSQTQKQEKLKTALVSPDYFNPFGNKLIQSGYIDKDQLKQALVEVRNQKKPLVKILEEMTQRALSPELVRQYKK
HHLFELKILYGVDCVDPESEEIASEQMTDLIESVVGIQICRRHKLLPLKRIEEDPPVLLIAMVNPDNLQALDDMKKLLRS
KNLGLQRIVITEEDYDKLLTKYFEQQQERQKLKEAKEKIQELERLGDVSGLVDDLGSNSLDEAEGEGGDDLASDSEANQA
PIIELVNKILIKAIQEGASDIHVEPQEENFRVRLRKDGVLRQYFTLPKHIIPAVTARFKIMGELDISERRLPQDGKIRRK
FQGRTVDFRMNTLPSRYGEKVCLRILDNSATQLGLNFLISDEETLAKVRDIASRPFGLILVTGPTGSGKSTSLYSVLAER
NEPGVNISTAEDPIEYALPGITQVQVIREKGMNFASILRAFMRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTN
DAAGAIARLDEMGVEPFMISGALLGVLAQRLMRRVCSECKEAYNPTREELARYGLLASNEESVTFYRAKSLKGEELQTAS
ANGTVCMKCGGTGYKGRVGVYEFMVMSERLEKLINQGATTDMIKEAAVEEGMTTILAYSLNLVRQGHTTLEEVERVTFTD
SGLEAELKAKRKTSLVCSNCDAELEQQWLDCPYCMTPRFGEAK

Nucleotide


Download         Length: 2052 bp        

>NTDB_id=66932 GM3708_RS11405 WP_066346987.1 2578279..2580330(-) (pilB/pilB1) [Geminocystis sp. NIES-3708]
ATGACTCAAACTCCTATTTCCTCACAAACCCAAAAACAAGAAAAACTCAAAACTGCTTTAGTTAGTCCAGATTATTTTAA
TCCCTTTGGCAATAAGTTAATTCAATCAGGTTATATCGATAAAGATCAATTGAAACAAGCTCTAGTAGAAGTTAGAAATC
AGAAAAAACCTCTTGTTAAAATATTGGAAGAAATGACTCAGAGAGCCTTATCTCCTGAGTTAGTGAGGCAATATAAAAAA
CATCATCTCTTTGAGTTAAAAATACTCTACGGAGTTGATTGTGTAGATCCTGAATCGGAAGAAATTGCTTCCGAACAAAT
GACGGATTTAATTGAATCAGTAGTAGGGATTCAAATTTGTCGTCGTCATAAATTATTACCTTTAAAACGCATTGAAGAAG
ATCCTCCTGTATTACTCATTGCCATGGTTAATCCTGATAATTTACAGGCTTTGGACGATATGAAAAAATTATTGCGTTCG
AAAAACTTAGGTTTACAGCGTATCGTAATTACGGAGGAAGATTACGATAAATTATTAACTAAGTATTTTGAACAGCAACA
AGAAAGACAAAAATTAAAAGAAGCGAAGGAGAAAATACAAGAGTTAGAAAGATTAGGAGATGTTAGTGGACTTGTCGATG
ATTTAGGTAGTAATAGTTTAGATGAAGCAGAAGGAGAAGGCGGAGATGATTTAGCCTCCGATAGTGAAGCAAACCAAGCA
CCCATAATTGAATTAGTTAATAAAATTTTAATTAAAGCCATACAAGAAGGTGCATCAGATATTCATGTTGAACCTCAAGA
AGAAAACTTCCGAGTACGTTTACGTAAAGATGGGGTTTTGCGACAGTATTTTACTTTGCCCAAACATATCATTCCCGCAG
TAACTGCTCGTTTCAAAATTATGGGGGAATTGGATATTTCTGAACGTCGTTTACCCCAAGATGGTAAAATTCGTCGCAAA
TTTCAGGGACGCACTGTCGATTTTCGGATGAATACTTTACCTAGTCGTTATGGTGAAAAAGTATGTTTACGTATTCTAGA
CAACTCTGCTACTCAATTAGGTTTAAACTTCCTCATCAGTGACGAAGAAACTTTAGCAAAAGTAAGAGATATTGCTAGTC
GTCCTTTTGGTTTAATTTTAGTTACAGGACCGACAGGCTCAGGTAAGTCCACCAGTTTATATTCAGTTTTAGCGGAAAGA
AATGAACCTGGAGTTAATATTAGCACGGCGGAAGATCCTATTGAGTATGCTTTACCCGGTATTACTCAAGTACAAGTAAT
TCGAGAAAAAGGCATGAACTTTGCTTCGATTCTACGGGCATTTATGCGTCAAGATCCTGATATTATTCTTGTGGGTGAAA
CTAGAGACGCAGAAACCGCCAAAACAGCTATTGAAGCAGCTTTAACTGGTCACTTAGTCTTAACAACTTTACATACTAAC
GATGCCGCAGGTGCGATCGCTCGTTTAGATGAAATGGGAGTTGAACCCTTTATGATTTCAGGAGCTTTACTGGGGGTTTT
GGCTCAACGTTTAATGCGTCGAGTGTGTAGTGAGTGTAAGGAAGCCTATAATCCTACAAGGGAAGAATTAGCCCGTTATG
GACTATTAGCATCTAATGAGGAATCAGTGACTTTTTATCGTGCCAAAAGTTTAAAAGGAGAAGAGCTACAAACAGCAAGT
GCTAATGGCACAGTTTGTATGAAGTGCGGTGGCACTGGTTATAAAGGAAGGGTTGGGGTATATGAATTTATGGTTATGAG
TGAAAGATTAGAAAAGCTGATCAATCAAGGGGCAACCACTGATATGATTAAAGAAGCTGCGGTAGAAGAGGGCATGACAA
CGATTTTGGCTTATAGTCTTAACTTAGTACGTCAAGGACATACAACTTTAGAAGAAGTGGAAAGGGTTACATTTACAGAC
TCGGGTTTAGAAGCTGAATTAAAAGCAAAACGTAAAACATCTTTAGTCTGCAGTAATTGTGATGCCGAACTAGAACAACA
ATGGTTGGATTGCCCTTACTGTATGACTCCTCGTTTTGGTGAAGCAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(248-514)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(33-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6AGM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.413

96.34

0.64
  pilF Thermus thermophilus HB27

39.437

93.558

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.49

91.947

0.363

Multiple sequence alignment