Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   H7K50_RS00360 Genome accession   NZ_AP022221
Coordinates   87019..89100 (-) Length   693 a.a.
NCBI ID   WP_000678399.1    Uniprot ID   -
Organism   Escherichia coli strain WP8-S17-ESBL-08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 82019..94100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K50_RS00345 (WP8S17E08_00670) yicH 82279..83988 (-) 1710 WP_001551854.1 AsmA family protein -
  H7K50_RS00350 (WP8S17E08_00680) xanP 84109..85500 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  H7K50_RS00355 (WP8S17E08_00690) gltS 85780..86985 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  H7K50_RS00360 (WP8S17E08_00700) recG 87019..89100 (-) 2082 WP_000678399.1 ATP-dependent DNA helicase RecG Machinery gene
  H7K50_RS00365 (WP8S17E08_00710) trmH 89106..89795 (-) 690 WP_001070187.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  H7K50_RS00370 (WP8S17E08_00720) spoT 89802..91910 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  H7K50_RS00375 (WP8S17E08_00730) rpoZ 91929..92204 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  H7K50_RS00380 (WP8S17E08_00740) gmk 92259..92882 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76479.42 Da        Isoelectric Point: 7.3384

>NTDB_id=66833 H7K50_RS00360 WP_000678399.1 87019..89100(-) (recG) [Escherichia coli strain WP8-S17-ESBL-08]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRNDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=66833 H7K50_RS00360 WP_000678399.1 87019..89100(-) (recG) [Escherichia coli strain WP8-S17-ESBL-08]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGTGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTACCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACCATGCGCTTTTTCAACTTCAACGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGCGTGCTGGCCTATGGAGAAGCAAAGCGCGGTAAATATGGCGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGTACGCCAGAGTTGCAGGAAACGCTTACGCCGGTTTACCCAACAACCGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCAGCACAACGCCGTCTGATTCTGGAAGAACTGCTTGCGCACAACCTCAGCATGTTGGCGTTACGC
GCTGGTGCACAACGTTTCCATGCCCAGCCGCTTAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCAGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTGGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGCGCCGATGATGCGCCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCGAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCGCGACTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGTCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCGACGCCGATCCCCCGCACGCTGGCGATGACCGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACGACAGTGGCAATCCCGGATACGCGTCGTAACGACATTATCGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTGTGCACACTCATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGTCTGGTACACGGACGGATGAAACCAGCCGAGAAGCAGG
CCGTCATGGCATCATTTAAACAAGGCGAGTTACACCTGCTGGTTGCCACTACCGTTATTGAAGTCGGCGTTGATGTGCCT
AACGCCAGCCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTGCACCAGCTTCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGGCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTGAGTTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAAGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.381

96.97

0.508
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.21

94.949

0.372

Multiple sequence alignment