Detailed information NTG0663211

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MNY35_RS18880
• Genome accession: NZ_CP093298
• Coordinates: 3872777..3873391 (+)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain MN-13
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3867777..3878391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MNY35_RS18845 (MNY35_18810)	aroE	3868464..3869306 (+)	843	WP_039254404.1	shikimate dehydrogenase	-
MNY35_RS18850 (MNY35_18815)	yhbY	3869300..3869590 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MNY35_RS18855 (MNY35_18820)	-	3869601..3870170 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MNY35_RS18860 (MNY35_18825)	yqeK	3870160..3870720 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MNY35_RS18865 (MNY35_18830)	rsfS	3870738..3871094 (+)	357	WP_007408260.1	ribosome silencing factor	-
MNY35_RS18870 (MNY35_18835)	-	3871091..3871828 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
MNY35_RS18875 (MNY35_18840)	comER	3871897..3872718 (-)	822	WP_039254409.1	late competence protein ComER	-
MNY35_RS18880 (MNY35_18845)	comEA	3872777..3873391 (+)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
MNY35_RS18885 (MNY35_18850)	-	3873458..3874027 (+)	570	WP_003152868.1	ComE operon protein 2	-
MNY35_RS18890 (MNY35_18855)	comEC	3874028..3876379 (+)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MNY35_RS18895 (MNY35_18860)	-	3876398..3876532 (-)	135	WP_003152870.1	YqzM family protein	-
MNY35_RS18900 (MNY35_18865)	-	3876573..3876724 (+)	152	Protein_3668	hypothetical protein	-
MNY35_RS18905 (MNY35_18870)	holA	3876764..3877805 (+)	1042	Protein_3669	DNA polymerase III subunit delta	-
MNY35_RS18910 (MNY35_18875)	rpsT	3877822..3878088 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=663211 MNY35_RS18880 WP_015240245.1 3872777..3873391(+) (comEA) [Bacillus amyloliquefaciens strain MN-13]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=663211 MNY35_RS18880 WP_015240245.1 3872777..3873391(+) (comEA) [Bacillus amyloliquefaciens strain MN-13]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363