Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MM522_RS10400 Genome accession   NZ_CP093067
Coordinates   2039050..2039667 (-) Length   205 a.a.
NCBI ID   WP_029317886.1    Uniprot ID   -
Organism   Bacillus subtilis strain ATC-3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034050..2044667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM522_RS10370 (MM522_10370) rpsT 2034352..2034618 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MM522_RS10375 (MM522_10375) holA 2034633..2035676 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  MM522_RS10380 (MM522_10380) - 2035716..2035865 (-) 150 WP_003229985.1 hypothetical protein -
  MM522_RS10385 (MM522_10385) yqzM 2035906..2036040 (+) 135 WP_003229983.1 YqzM family protein -
  MM522_RS10390 (MM522_10390) comEC 2036080..2038410 (-) 2331 WP_101172381.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MM522_RS10395 (MM522_10395) comEB 2038414..2038983 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MM522_RS10400 (MM522_10400) comEA 2039050..2039667 (-) 618 WP_029317886.1 competence protein ComEA Machinery gene
  MM522_RS10405 (MM522_10405) comER 2039751..2040572 (+) 822 WP_014480313.1 late competence protein ComER -
  MM522_RS10410 (MM522_10410) yqeM 2040638..2041381 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  MM522_RS10415 (MM522_10415) rsfS 2041378..2041734 (-) 357 WP_014480315.1 ribosome silencing factor -
  MM522_RS10420 (MM522_10420) yqeK 2041752..2042312 (-) 561 WP_029317884.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MM522_RS10425 (MM522_10425) nadD 2042302..2042871 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MM522_RS10430 (MM522_10430) yhbY 2042883..2043173 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MM522_RS10435 (MM522_10435) aroE 2043167..2044009 (-) 843 WP_101172380.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21722.44 Da        Isoelectric Point: 4.7220

>NTDB_id=661852 MM522_RS10400 WP_029317886.1 2039050..2039667(-) (comEA) [Bacillus subtilis strain ATC-3]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=661852 MM522_RS10400 WP_029317886.1 2039050..2039667(-) (comEA) [Bacillus subtilis strain ATC-3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGGCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38