Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MK848_RS05070 Genome accession   NZ_CP092824
Coordinates   1024052..1024669 (-) Length   205 a.a.
NCBI ID   WP_029317886.1    Uniprot ID   -
Organism   Bacillus subtilis strain S1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019052..1029669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS05040 (MK848_05045) rpsT 1019354..1019620 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MK848_RS05045 (MK848_05050) holA 1019635..1020678 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  MK848_RS05050 (MK848_05055) - 1020718..1020867 (-) 150 WP_003229985.1 hypothetical protein -
  MK848_RS05055 (MK848_05060) yqzM 1020908..1021042 (+) 135 WP_003229983.1 YqzM family protein -
  MK848_RS05060 (MK848_05065) comEC 1021082..1023412 (-) 2331 WP_101172381.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MK848_RS05065 (MK848_05070) comEB 1023416..1023985 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MK848_RS05070 (MK848_05075) comEA 1024052..1024669 (-) 618 WP_029317886.1 competence protein ComEA Machinery gene
  MK848_RS05075 (MK848_05080) comER 1024753..1025574 (+) 822 WP_014480313.1 late competence protein ComER -
  MK848_RS05080 (MK848_05085) yqeM 1025640..1026383 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  MK848_RS05085 (MK848_05090) rsfS 1026380..1026736 (-) 357 WP_014480315.1 ribosome silencing factor -
  MK848_RS05090 (MK848_05095) yqeK 1026754..1027314 (-) 561 WP_029317884.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MK848_RS05095 (MK848_05100) nadD 1027304..1027873 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MK848_RS05100 (MK848_05105) yhbY 1027885..1028175 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MK848_RS05105 (MK848_05110) aroE 1028169..1029011 (-) 843 WP_101172380.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21722.44 Da        Isoelectric Point: 4.7220

>NTDB_id=660419 MK848_RS05070 WP_029317886.1 1024052..1024669(-) (comEA) [Bacillus subtilis strain S1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=660419 MK848_RS05070 WP_029317886.1 1024052..1024669(-) (comEA) [Bacillus subtilis strain S1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGGCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38