Detailed information NTG0659973

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: MK801_RS06510
• Genome accession: NZ_CP092748
• Coordinates: 1285652..1286974 (+)
• Length: 440 a.a.
• NCBI ID: WP_128267736.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain 17M1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1280652..1291974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK801_RS06485 (MK801_06480)	glyQ	1281168..1282121 (-)	954	WP_057720279.1	glycine--tRNA ligase subunit alpha	-
MK801_RS06490 (MK801_06485)	-	1282407..1283153 (-)	747	WP_057720280.1	bifunctional metallophosphatase/5'-nucleotidase	-
MK801_RS06495 (MK801_06490)	-	1283268..1284287 (-)	1020	WP_057720281.1	DUF475 domain-containing protein	-
MK801_RS06500 (MK801_06495)	-	1284335..1284832 (-)	498	WP_254255446.1	VanZ family protein	-
MK801_RS06505 (MK801_06500)	-	1284966..1285595 (-)	630	WP_128267737.1	YigZ family protein	-
MK801_RS06510 (MK801_06505)	comFA	1285652..1286974 (+)	1323	WP_128267736.1	DEAD/DEAH box helicase	Machinery gene
MK801_RS06515 (MK801_06510)	-	1286971..1287621 (+)	651	WP_057720285.1	ComF family protein	-
MK801_RS06520 (MK801_06515)	-	1287658..1288233 (-)	576	WP_057720286.1	nucleotidyltransferase family protein	-
MK801_RS06525 (MK801_06520)	-	1288247..1288702 (-)	456	WP_003130653.1	diacylglycerol kinase family protein	-
MK801_RS06530 (MK801_06525)	ybeY	1288686..1289174 (-)	489	WP_057720287.1	rRNA maturation RNase YbeY	-
MK801_RS06535 (MK801_06530)	-	1289326..1289805 (-)	480	WP_003130650.1	NUDIX hydrolase	-
MK801_RS06540 (MK801_06535)	-	1289863..1290831 (-)	969	WP_003130649.1	PhoH family protein	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50260.83 Da
• Isoelectric Point: 10.1770

>NTDB_id=659973 MK801_RS06510 WP_128267736.1 1285652..1286974(+) (comFA) [Lactococcus lactis strain 17M1]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETTVKSILGMTLISSNKIKCNRCGTVHIKNSVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKSFPKKSYINWSGKLTENQKSVSNTLCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKNIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFYKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=659973 MK801_RS06510 WP_128267736.1 1285652..1286974(+) (comFA) [Lactococcus lactis strain 17M1]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACAACAGTAAAATCAATTTTGGGGATGACTTTAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATTAAAAATTCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAGTTTTCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAGTTTCAAATACCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTTCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGTTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAAATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTTATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-232)

• Helicase conserved C-terminal domain (Helicase_C) (297-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.25	90.909	0.675
comFA/cflA	Streptococcus pneumoniae Rx1	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae D39	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae R6	53.015	90.455	0.48
comFA/cflA	Streptococcus pneumoniae TIGR4	53.015	90.455	0.48
comFA/cflA	Streptococcus mitis SK321	52.513	90.455	0.475
comFA/cflA	Streptococcus mitis NCTC 12261	51.508	90.455	0.466
comFA	Bacillus subtilis subsp. subtilis str. 168	38.249	98.636	0.377