Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   MKW31_RS17795 Genome accession   NZ_CP092715
Coordinates   3644252..3644371 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain 37-1     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3639252..3649371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKW31_RS17775 (MKW31_17720) - 3639602..3640114 (+) 513 Protein_3440 bacteriocin biosynthesis protein AlbG -
  MKW31_RS17780 (MKW31_17725) - 3640121..3641497 (-) 1377 WP_349403722.1 YncE family protein -
  MKW31_RS17785 (MKW31_17730) - 3641537..3642892 (-) 1356 WP_053104069.1 YncE family protein -
  MKW31_RS17790 (MKW31_17735) rapF 3643123..3644268 (+) 1146 WP_327972459.1 tetratricopeptide repeat protein Regulator
  MKW31_RS17795 (MKW31_17740) phrF 3644252..3644371 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  MKW31_RS17800 (MKW31_17745) - 3644569..3644772 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  MKW31_RS17805 (MKW31_17750) - 3644786..3644989 (+) 204 WP_007407641.1 hypothetical protein -
  MKW31_RS17810 (MKW31_17755) - 3645024..3645209 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  MKW31_RS17815 (MKW31_17760) - 3645395..3645676 (+) 282 WP_007407643.1 hypothetical protein -
  MKW31_RS17820 (MKW31_17765) - 3645720..3646232 (+) 513 WP_039253458.1 stage II sporulation protein M -
  MKW31_RS17825 (MKW31_17770) - 3646823..3647041 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  MKW31_RS17830 (MKW31_17775) speB 3647209..3648081 (-) 873 WP_007614448.1 agmatinase -
  MKW31_RS17835 (MKW31_17780) speE 3648140..3648970 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=659876 MKW31_RS17795 WP_007407639.1 3644252..3644371(+) (phrF) [Bacillus velezensis strain 37-1]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=659876 MKW31_RS17795 WP_007407639.1 3644252..3644371(+) (phrF) [Bacillus velezensis strain 37-1]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462