Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MKI66_RS07495 Genome accession   NZ_CP092547
Coordinates   1567636..1568133 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VMRSA-WC083     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1562636..1573133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI66_RS07470 (MKI66_001474) gcvPB 1562800..1564272 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  MKI66_RS07475 (MKI66_001475) gcvPA 1564265..1565611 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  MKI66_RS07480 (MKI66_001476) gcvT 1565631..1566722 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  MKI66_RS07485 (MKI66_001477) - 1566881..1567405 (-) 525 WP_001015120.1 shikimate kinase -
  MKI66_RS07490 (MKI66_001478) - 1567395..1567541 (-) 147 WP_001792109.1 hypothetical protein -
  MKI66_RS07495 (MKI66_001479) comGF 1567636..1568133 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  MKI66_RS07500 (MKI66_001480) - 1568051..1568351 (-) 301 Protein_1472 competence protein ComGE -
  MKI66_RS07505 (MKI66_001481) comGD 1568338..1568780 (-) 443 Protein_1473 competence type IV pilus minor pilin ComGD -
  MKI66_RS07510 (MKI66_001482) comGC 1568758..1569069 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MKI66_RS07515 (MKI66_001483) comGB 1569083..1570149 (-) 1067 Protein_1475 competence type IV pilus assembly protein ComGB -
  MKI66_RS07520 (MKI66_001484) comGA 1570121..1571095 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MKI66_RS07525 (MKI66_001485) - 1571147..1571770 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MKI66_RS07530 (MKI66_001486) - 1571767..1572096 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MKI66_RS07535 (MKI66_001487) - 1572096..1573082 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=658598 MKI66_RS07495 WP_001788897.1 1567636..1568133(-) (comGF) [Staphylococcus aureus strain VMRSA-WC083]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=658598 MKI66_RS07495 WP_001788897.1 1567636..1568133(-) (comGF) [Staphylococcus aureus strain VMRSA-WC083]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964