Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKI66_RS07520 Genome accession   NZ_CP092547
Coordinates   1570121..1571095 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain VMRSA-WC083     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1565121..1576095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI66_RS07480 (MKI66_001476) gcvT 1565631..1566722 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  MKI66_RS07485 (MKI66_001477) - 1566881..1567405 (-) 525 WP_001015120.1 shikimate kinase -
  MKI66_RS07490 (MKI66_001478) - 1567395..1567541 (-) 147 WP_001792109.1 hypothetical protein -
  MKI66_RS07495 (MKI66_001479) comGF 1567636..1568133 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  MKI66_RS07500 (MKI66_001480) - 1568051..1568351 (-) 301 Protein_1472 competence protein ComGE -
  MKI66_RS07505 (MKI66_001481) comGD 1568338..1568780 (-) 443 Protein_1473 competence type IV pilus minor pilin ComGD -
  MKI66_RS07510 (MKI66_001482) comGC 1568758..1569069 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  MKI66_RS07515 (MKI66_001483) comGB 1569083..1570149 (-) 1067 Protein_1475 competence type IV pilus assembly protein ComGB -
  MKI66_RS07520 (MKI66_001484) comGA 1570121..1571095 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MKI66_RS07525 (MKI66_001485) - 1571147..1571770 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MKI66_RS07530 (MKI66_001486) - 1571767..1572096 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MKI66_RS07535 (MKI66_001487) - 1572096..1573082 (-) 987 WP_000161311.1 ROK family glucokinase -
  MKI66_RS07540 (MKI66_001488) - 1573079..1573282 (-) 204 WP_000087561.1 YqgQ family protein -
  MKI66_RS07545 (MKI66_001489) - 1573263..1574726 (-) 1464 WP_001017857.1 rhomboid family intramembrane serine protease -
  MKI66_RS07550 (MKI66_001490) - 1574738..1575277 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  MKI66_RS07555 (MKI66_001491) rpmG 1575486..1575635 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=658600 MKI66_RS07520 WP_000697220.1 1570121..1571095(-) (comGA) [Staphylococcus aureus strain VMRSA-WC083]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=658600 MKI66_RS07520 WP_000697220.1 1570121..1571095(-) (comGA) [Staphylococcus aureus strain VMRSA-WC083]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1